Department of Physiology and Pharmaceutical Research Centre, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran.
Evid Based Complement Alternat Med. 2011;2011:857124. doi: 10.1155/2011/857124. Epub 2010 Dec 1.
The stimulatory effect of Zataria multiflora Boiss (Labiatae) and carvacrol on β-adrenoceptors was examined on guinea pig trachea. The effects of three concentrations of aqueous-ethanolic extract, carvacrol, and propranolol (β-receptor antagonist) on β-adrenoceptors were tested in nonincubated (group 1, n = 8) and incubated tracheal chains with 1 μM chlorpheniramine (histamine H1 receptor antagonist) (group 2, n = 5). Isoprenaline (β-receptor agonist) curves obtained in the presence of all concentrations of the extract and carvacrol showed leftward shifts compared with that of saline in both groups. In both groups, the EC50 (the effective concentration of isoprenaline, causing 50% of maximum response) obtained in the presence of all concentrations of the extract and carvacrol was significantly lower compared to that of saline (P < .01 to P < .001). All values of (CR-1: (EC50 in the presence of active substances/EC50 obtained in the presence of saline)-1) obtained in the presence of concentrations of the extract and carvacrol in both groups were negative and significantly different from that of propranolol (P < .001 for all cases). The results indicated a stimulatory effect of Zataria multiflora Boiss extract on β(2)-adrenoceptors which is perhaps due to its constituent, carvacrol.
百里香（唇形科）和香芹酚对豚鼠气管β-肾上腺素能受体的刺激作用。在未孵育（第 1 组，n = 8）和孵育（第 2 组，n = 5）的气管链中，测试了三种浓度的水-乙醇提取物、香芹酚和普萘洛尔（β-受体拮抗剂）对β-肾上腺素能受体的影响。与生理盐水相比，在所有提取物和香芹酚浓度存在的情况下获得的异丙肾上腺素（β-受体激动剂）曲线在两组中均向左移位。在两组中，与生理盐水相比，在所有提取物和香芹酚浓度存在的情况下获得的 EC50（异丙肾上腺素的有效浓度，引起最大反应的 50%）均显著降低（P <.01 至 P <.001）。在两组中，在存在提取物和香芹酚浓度的情况下获得的所有（CR-1：（存在活性物质的 EC50/存在生理盐水的 EC50）-1）值均为负，且与普萘洛尔显著不同（所有情况下 P <.001）。结果表明，百里香提取物对β（2）-肾上腺素能受体具有刺激作用，这可能是由于其成分香芹酚所致。
