Forde Aaron J, Albrecht Nadine, Klingl Andreas, Donovan Catriona, Bramkamp Marc
Fakultät für Biologie, Ludwig-Maximilians-Universität MünchenMunich, Germany.
Front Microbiol. 2017 Aug 2;8:1429. doi: 10.3389/fmicb.2017.01429. eCollection 2017.
The ATCC 13032 prophage CGP3 encodes an actin-like protein, AlpC that was shown to be involved in viral DNA transport and efficient viral DNA replication. AlpC binds to an adapter, AlpA that in turn binds to specific DNA sequences, termed sites. Thus, the AlpAC system is similar to the known plasmid segregation system ParMRS. So far it is unclear how the AlpACS system mediates DNA transport and, whether AlpA and AlpC functionally interact. We show here that AlpA modulates AlpC filamentation dynamics in a dual way. Unbound AlpA stimulates AlpC filament disassembly, while AlpA bound to sites allows for AlpC filament formation. Based on these results we propose a simple search and capture model that explains DNA segregation by viral AlpACS DNA segregation system.
ATCC 13032 前噬菌体CGP3编码一种肌动蛋白样蛋白AlpC，已证明其参与病毒DNA转运和高效病毒DNA复制。AlpC与衔接蛋白AlpA结合，而AlpA又与特定DNA序列（称为位点）结合。因此，AlpAC系统类似于已知的质粒分离系统ParMRS。到目前为止，尚不清楚AlpACS系统如何介导DNA转运，以及AlpA和AlpC在功能上是否相互作用。我们在此表明，AlpA以双重方式调节AlpC的丝状体形成动态。未结合的AlpA刺激AlpC丝状体解体，而与位点结合的AlpA则允许AlpC丝状体形成。基于这些结果，我们提出了一个简单的搜索和捕获模型，该模型解释了病毒AlpACS DNA分离系统的DNA分离过程。
