棒状杆菌中的原噬菌体分布

Prophage distribution in coryneform bacteria.

作者信息

Moreau S, Leret V, Le Marrec C, Varangot H, Ayache M, Bonnassie S, Blanco C, Trautwetter A

机构信息

Laboratoire de Génétique et Physiologie microbiennes, Rennes, France.

出版信息

Res Microbiol. 1995 Jul-Aug;146(6):493-505. doi: 10.1016/0923-2508(96)80295-6.

DOI:10.1016/0923-2508(96)80295-6

PMID:8525066

Abstract

Four temperate bacteriophages of corynebacteria were isolated after UV induction. Phages phi 304L and phi 304S were both induced from Corynebacterium glutamicum ATCC 13058, ATCC 21488, ATCC 21649 and ATCC 21650 strains, and have no known sensitive host. Phages phi 15 and phi 16 were both induced from ATCC 14020 and ATCC 21792. Phage phi 15 formed turbid plaques on Corynebacterium sp. ATCC 21857 and on C. glutamicum ATCC 13058, ATCC 21488, ATCC 21649 and ATCC 21650. Phage phi 16 produced turbid plaques only on C. glutamicum ATCC 21792 cured of prophage phi 16. All these phages belong to the Siphoviridae family. Their genomes consist of a double-stranded DNA with cohesive ends and share no homology with each other. Prophages phi 16, phi 304L and phi 304S were integrated into their respective host chromosomes, whereas prophage phi 15 seemed to persist free in the cell. Cross-hybridizations between phage DNAs and total cellular DNA obtained from 20 strains belonging to the genera Corynebacterium and Brevibacterium did not show the presence of these prophages in strains other than their respective hosts.

摘要

经紫外线诱导后，分离出了4株棒状杆菌的温和噬菌体。噬菌体phi 304L和phi 304S均从谷氨酸棒杆菌ATCC 13058、ATCC 21488、ATCC 21649和ATCC 21650菌株中诱导产生，且没有已知的敏感宿主。噬菌体phi 15和phi 16均从ATCC 14020和ATCC 21792中诱导产生。噬菌体phi 15在棒状杆菌属ATCC 21857以及谷氨酸棒杆菌ATCC 13058、ATCC 21488、ATCC 21649和ATCC 21650上形成浑浊噬菌斑。噬菌体phi 16仅在已去除原噬菌体phi 16的谷氨酸棒杆菌ATCC 21792上产生浑浊噬菌斑。所有这些噬菌体都属于长尾噬菌体科。它们的基因组由具有粘性末端的双链DNA组成，彼此之间没有同源性。原噬菌体phi 16、phi 304L和phi 304S整合到了它们各自的宿主染色体中，而原噬菌体phi 15似乎在细胞中以游离状态存在。从属于棒状杆菌属和短杆菌属的20个菌株中获得的噬菌体DNA与总细胞DNA之间的交叉杂交结果表明，除了各自的宿主菌株外，其他菌株中不存在这些原噬菌体。