Inositol Phosphorylceramide: Distinctive Sphingoid Base Composition.

Inositol phosphorylceramide (IPC), the major sphingolipid in the genus but not found in mammals, is considered a potentially useful target for chemotherapy against leishmaniasis. is endemic in Latin America and causes American tegumentary leishmaniasis. We demonstrated that IPCs are localized internally in parasites, using a specific monoclonal antibody. Treatment with 5 μM myriocin (a serine palmitoyltransferase inhibitor) rendered promastigotes 8-fold less infective than controls in experimental hamster infection, as determined by number of parasites per inguinal lymph node after 8 weeks infection, suggesting the importance of parasite IPC or sphingolipid derivatives in parasite infectivity or survival in the host. IPC was isolated from promastigotes of three strains and analyzed by positive- and negative-ion ESI-MS. The major IPC ions were characterized as eicosasphinganine and eicosasphingosine. Negative-ion ESI-MS revealed IPC ion species at 778.6 (d20:1/14:0), 780.6 (d20:0/14:0), 796.6 (t20:0/14:0), 806.6 (d20:1/16:0), and 808.6 (d20:0/16:0). IPCs isolated from and showed significant differences in IPC ceramide composition. The major IPC ion from , detected in negative-ion ESI-MS at 780.6, was composed of ceramide d16:1/18:0. Our results suggest that sphingosine synthase (also known as serine palmitoyltransferase; SPT) in is responsible for synthesis of a long-chain base of 20 carbons (d20), whereas SPT in synthesizes a 16-carbon long-chain base (d16). A phylogenetic tree based on SPT proteins was constructed by analysis of sequence homologies in species of the and subgenera. Results indicate that SPT gene position in s is completely separated from that of members of subgenus , including , and . Our findings clearly demonstrate sphingoid base differences between and members of subgenus , and are relevant to future development of more effective targeted anti-leishmaniasis drugs.