Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
Research Center for Industrial Developement of Seafood, Gyeongsang National University, Republic of Korea.
Int J Biol Macromol. 2018 Jan;106:330-337. doi: 10.1016/j.ijbiomac.2017.08.027. Epub 2017 Aug 5.
Heavy metal contamination has become a major problem that causes severe environmental and health issues due to their biosorption, bioaccumulation, and toxicity. This study was designed to evaluate heavy metal chelating abilities of alginic acid (AA) extracted from the brown seaweed Ecklonia cava and two of its derivatives prepared by the partial oxidation of the 2° OH groups (OAA) and partial carboxylation of the monomeric units (CAA) upon reducing the heavy metal biosorption in zebrafish (Danio rerio) modal. Metal ions were quantified using ICP-OES and biopolymers were characterized by FTIR and XRD analysis. All investigated biopolymers indicated potential ability for chelating Pb, Cu, Cd, As, and Ag. The sorption capacities were in the order of CAA>OAA>AA. All biopolymers indicated a comparatively higher chelation towards Pb. AA, OAA, and CAA could effectively reduce Pb induced toxicity and Pb stress-induced ROS production in zebrafish embryos. Besides, they could reduce the biosorption of Pb in adult zebrafish which could lead to bioaccumulation. Since alginic acid purified from E. cava and its derivatives could be utilized as seaweed derived biopolymers to purify heavy metals contaminated water and as a dietary supplement to reduce heavy metal biosorption in organisms.
重金属污染已成为一个主要问题，由于其生物吸附、生物积累和毒性，会导致严重的环境和健康问题。本研究旨在评估从褐藻裙带菜中提取的海藻酸（AA）及其两种衍生物（通过部分氧化 2°OH 基团（OAA）和单体单元部分羧化（CAA）制备）对斑马鱼（Danio rerio）模式的重金属吸附的螯合能力。使用 ICP-OES 定量金属离子，并用 FTIR 和 XRD 分析对生物聚合物进行表征。所有研究的生物聚合物均表明对 Pb、Cu、Cd、As 和 Ag 具有潜在的螯合能力。螯合能力的顺序为 CAA＞OAA＞AA。所有生物聚合物均表明对 Pb 的螯合作用较强。AA、OAA 和 CAA 可有效降低 Pb 诱导的斑马鱼胚胎毒性和 Pb 应激诱导的 ROS 产生。此外，它们还可以减少成年斑马鱼对 Pb 的生物吸附，从而导致生物积累。由于从裙带菜中纯化的海藻酸及其衍生物可用作从受污染水中纯化重金属的海藻衍生生物聚合物，以及作为减少生物体内重金属吸附的膳食补充剂。
