Department of Oncology, Affiliated Hospital of South China Sea, Southern Medical University, Foshan 528200, P.R. China.
Department of Respiratory Medicine, Affiliated Hospital of South China Sea, Southern Medical University, Foshan 528200, P.R. China
Biosci Rep. 2017 Nov 6;37(6). doi: 10.1042/BSR20171086. Print 2017 Dec 22.
Lung cancer is one of the most common malignant tumors harmful to human health. Cytokeratin (CK) is highly conserved and differentiated related to the proliferation and differentiation of epithelial cells. The aim of the study was to explore expressions of CK20 and CK7 and corresponding prognostic values in patients with lung cancer. Our study included 258 cases of patients confirmed with lung cancer. Expressions of and mRNA and protein were detected using real-time quantitative PCR (qRT-PCR) and Western blot, respectively, followed by the performance of immunohistochemistry staining. Associations of CK20 and CK7 with the clinical parameters and prognosis of lung cancer patients were further analyzed. There were obvious differences regarding the positive expression of CK20 in different T stage, lymph node metastasis, invasion, size, and clinical stage subgroups; besides, significant differences in the positive expression of CK7 were also observed in subgroups of different sex, age, lymph node metastasis, invasion, and differentiation. Furthermore, effects of age, smoking, T stage, lymph node metastasis and invasion, size, and CK7 expressions were significant on the survival of patients (all <0.05). Multivariate analysis revealed that lymph node metastasis, T stage, and CK7 expression were independent risk factors for poor prognosis of involved patients (all <0.05), while age, smoking, and invasion had no marked relation to the survival time of patients with lung cancer (all >0.05). Positive CK20 and CK7 expressions are detected in patients with lung cancer; positive expression of CK7 associated with pathological features of lymph node metastasis and T stage may be independent clinical parameters for poor prognosis of patients with lung cancer.
肺癌是对人类健康危害最大的常见恶性肿瘤之一。细胞角蛋白（CK）与上皮细胞的增殖和分化密切相关，高度保守和分化。本研究旨在探讨 CK20 和 CK7 在肺癌患者中的表达及其与预后的关系。本研究纳入 258 例经病理证实的肺癌患者，采用实时定量 PCR（qRT-PCR）和 Western blot 分别检测 CK20 和 CK7 mRNA 和蛋白的表达，随后进行免疫组织化学染色。进一步分析 CK20 和 CK7 与肺癌患者临床参数和预后的关系。不同 T 分期、淋巴结转移、浸润、大小和临床分期亚组 CK20 阳性表达存在明显差异；不同性别、年龄、淋巴结转移、浸润和分化亚组 CK7 阳性表达也存在明显差异。此外，年龄、吸烟、T 分期、淋巴结转移和浸润、大小和 CK7 表达对患者的生存有显著影响（均<0.05）。多因素分析显示，淋巴结转移、T 分期和 CK7 表达是影响患者预后的独立危险因素（均<0.05），而年龄、吸烟和浸润与肺癌患者的生存时间无明显关系（均>0.05）。肺癌患者 CK20 和 CK7 阳性表达；CK7 阳性表达与淋巴结转移和 T 分期的病理特征相关，可能是肺癌患者预后不良的独立临床参数。
