MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, China.
University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Sci Rep. 2017 Aug 21;7(1):8335. doi: 10.1038/s41598-017-07798-1.
Numerous studies have examined the prevalence of pathogenic Escherichia coli in poultry and poultry products; however, limited data are available regarding their resistance- and virulence-associated gene expression profiles. This study was designed to examine the resistance and virulence of poultry E. coli strains in vitro and in vivo via antibiotic susceptibility, biofilm formation and adhesion, and invasion and intracellular survivability assays in Caco-2 and Raw 264.7 cell lines as well as the determination of the median lethal dose in two-day old chickens. A clinical pathogenic multidrug-resistant isolate, E. coli 381, isolated from broilers, was found to be highly virulent in cell culture and 1000-fold more virulent in a chicken model than other strains; accordingly, the isolate was subsequently selected for transcriptome analysis. The comparative gene expression profile of MDR E. coli 381 and the reference human strain E. coli ATCC 25922 was completed with Illumina HiSeq. 2500 transcriptome analysis. Differential gene expression analysis indicates that there are multiple pathways involved in the resistance and virulence of this highly virulent strain. The results garnered from this study provide critical information about the highly virulent MDR E. coli strain of poultry origin and warrant further investigation due to its significant threat to public health.
许多研究都调查了禽肉和禽产品中致病性大肠杆菌的流行情况；然而，关于其耐药性和毒力相关基因表达谱的数据有限。本研究旨在通过体外和体内抗生素药敏试验、生物膜形成和黏附试验、Caco-2 和 Raw 264.7 细胞系的侵袭和细胞内存活能力试验以及在两天大的鸡中的半数致死量测定，来检测禽源大肠杆菌菌株的耐药性和毒力。从肉鸡中分离出的临床致病性多药耐药分离株大肠杆菌 381 在细胞培养中具有高度毒力，在鸡模型中比其他菌株毒力高 1000 倍；因此，该分离株随后被选择进行转录组分析。通过 Illumina HiSeq 2500 转录组分析完成了 MDR 大肠杆菌 381 和参考人源菌株大肠杆菌 ATCC 25922 的比较基因表达谱。差异基因表达分析表明，该高毒力菌株的耐药性和毒力涉及多个途径。本研究的结果提供了关于高毒力禽源 MDR 大肠杆菌菌株的重要信息，由于其对公共卫生的重大威胁，值得进一步研究。
