Potter A A, Ready K, Gilchrist J
Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.
Microb Pathog. 1988 Apr;4(4):311-6. doi: 10.1016/0882-4010(88)90092-7.
Pasteurella haemolytica A-1 produces large, rigid fimbriae with a diameter of 12 nm and a denisty of 1.32. Their subunit molecular weight was 35,000 as determined by SDS-polyacrylamide gel electrophoresis and immunoblotting with monoclonal antibodies raised against native fimbriae. The isoelectric point of the purified fimbriae was 4.8. While Pasteurella haemolytica whole cells plus a crude shear fraction were capable of agglutinating bovine erythrocytes, purified fimbriae exhibited no such activity.
溶血巴斯德菌A-1产生直径为12纳米、密度为1.32的大型刚性菌毛。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳以及用针对天然菌毛产生的单克隆抗体进行免疫印迹法测定，其亚基分子量为35,000。纯化菌毛的等电点为4.8。虽然溶血巴斯德菌全细胞加粗剪切级分能够凝集牛红细胞，但纯化菌毛没有这种活性。
