Manafi Abbas, Khodabandehloo Mazaher, Rouhi Samaneh, Ramazanzadeh Rashid, Shahbazi Babak, Narenji Hanar
Form the Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Adv Biomed Res. 2017 Jul 25;6:87. doi: 10.4103/abr.abr_243_15. eCollection 2017.
strains that are Panton-Valentine leukocidin (PVL) positive cause severe skin and soft tissue infections as well as necrotizing pneumonia. The presence of PVL gene is a marker for methicillin-resistant ; therefore, survey on prevalence and phylogenetic distribution of PVL is of great importance for public health. The aim of this research was molecular epidemiology survey of PVL positive, isolated from two tertiary hospitals of Sanandaj.
A total of 264 staphylococci isolates were collected from clinical specimens, hospital personnel and hospital environment of two tertiary hospitals of Sanandaj, in 2012 (Toohid and Besat). Bacterial cultures and biochemical tests were performed for detection. Then, polymerase chain reaction (PCR) and repetitive sequence-based PCR (rep-PCR) were used for the determination of prevalence and molecular epidemiology of PVL, respectively. Data were analyzed using the Fisher's exact test ( < 0.05).
From 264 staphylococci isolates, 88 (33.33%) were detected as . Furthermore, 20 out of 88 (22.72%) strains of were PVL positive according to PCR results. Rep-PCR showed six main clusters of samples. PVL had similar clonality between different samples. No significant relationship was observed between PVL positive and rep-PCR patterns ( = 0.98).
These results showed that a clone of PVL positive has spread between the community and hospital settings. Therefore, appropriate measures are required to prevent the spread of staphylococci and other bacteria in hospitals.
携带杀白细胞毒素（PVL）的菌株可引起严重的皮肤和软组织感染以及坏死性肺炎。PVL基因的存在是耐甲氧西林的一个标志；因此，对PVL的流行情况和系统发育分布进行调查对公共卫生至关重要。本研究的目的是对从萨南达杰的两家三级医院分离出的PVL阳性菌株进行分子流行病学调查。
2012年从萨南达杰的两家三级医院（图希德医院和贝萨特医院）的临床标本、医院工作人员和医院环境中总共收集了264株葡萄球菌分离株。进行细菌培养和生化试验以进行检测。然后，分别使用聚合酶链反应（PCR）和基于重复序列的PCR（rep-PCR）来确定PVL阳性菌株的流行情况和分子流行病学。使用Fisher精确检验（P<0.05）分析数据。
在264株葡萄球菌分离株中，检测到88株（33.33%）为[未提及具体菌名]。此外，根据PCR结果，88株[未提及具体菌名]菌株中有20株（22.72%）PVL呈阳性。rep-PCR显示[未提及具体菌名]样本有六个主要聚类。PVL在不同样本之间具有相似的克隆性。未观察到PVL阳性[未提及具体菌名]与rep-PCR模式之间存在显著关系（P = 0.98）。
这些结果表明，一株PVL阳性的[未提及具体菌名]克隆已在社区和医院环境之间传播。因此，需要采取适当措施来防止葡萄球菌和其他细菌在医院传播。
