Havaei Sa, Moghadam S Ohadian, Pourmand Mr, Faghri J
Dept. of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Iran.
Iran J Public Health. 2010;39(1):8-14. Epub 2010 Mar 31.
Staphylococcus aureus has been recognized as a major human pathogen and is the major cause of nosocomial infections. Gamma-toxin, leukocidin and other bi-component toxins are a family of proteins encoded by the hlg and luk-PV, respectively. Panton-Valentine leukocidin (PVL) is an example of these toxins and causes leukocyte destruction and tissue necrosis. The aim of this study was to determine the prevalence of bi-component leukocidin in Methicillin Resistant Staphylococcus aureus (MRSA) isolates in staphylococcal infections.
Collectively, 143 isolates of S. aureus were obtained from Tehran University of Medical Sciences hospitals and confirmed with biochemical tests. Then polymerase chain reaction was used to detect luk-PV loci and luk-E/D. Coagulase gene was used as internal control. The antibiotic susceptibility patterns of isolates were determined using disk diffusion method.
Out of 149 S. aureus isolates 24.2% were luk-PV positive and 73.8% were luk-E/D positive.
There was PVL positive MRSA isolates with high prevalence in evaluated hospitals. The diseases from these bacteria are with extensive necrosis, leucopenia and even death. We desire that, prevent from progress and death by diagnosis and right treatment.
金黄色葡萄球菌已被公认为主要的人类病原体，是医院感染的主要原因。γ毒素、杀白细胞素及其他双组分毒素分别是由hlg和luk-PV编码的一类蛋白质。Panton-Valentine杀白细胞素（PVL）就是这些毒素的一种，可导致白细胞破坏和组织坏死。本研究旨在确定双组分杀白细胞素在葡萄球菌感染的耐甲氧西林金黄色葡萄球菌（MRSA）分离株中的流行情况。
共从德黑兰医科大学附属医院获得143株金黄色葡萄球菌分离株，并通过生化试验进行确认。然后采用聚合酶链反应检测luk-PV基因座和luk-E/D。凝固酶基因用作内对照。采用纸片扩散法测定分离株的抗生素敏感性模式。
在149株金黄色葡萄球菌分离株中，24.2%的luk-PV呈阳性，73.8%的luk-E/D呈阳性。
在所评估的医院中，PVL阳性的MRSA分离株具有较高的流行率。这些细菌引起的疾病会导致广泛坏死、白细胞减少甚至死亡。我们希望通过诊断和正确治疗来预防病情进展和死亡。
