Wang Xin
Center for Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Suite R10, Houston, TX, 77030, USA.
Methods Mol Biol. 2017;1660:351-353. doi: 10.1007/978-1-4939-7253-1_28.
Isolation of extracellular vesicles (EVs) from breast milk enables the downstream analysis of the contents, including various proteins, lipids, and RNAs (messenger RNAs and microRNAs). Here, we present the method of centrifugation with ExoQuick™ precipitation to isolate EVs from breast milk. It has been proven to be a rapid and effective alternative compared to conventional differential centrifugation method.
从母乳中分离细胞外囊泡（EVs）能够对其内容物进行下游分析，这些内容物包括各种蛋白质、脂质和RNA（信使RNA和微小RNA）。在此，我们介绍一种使用ExoQuick™沉淀法通过离心从母乳中分离EVs的方法。与传统的差速离心法相比，该方法已被证明是一种快速有效的替代方法。
