新型分枝杆菌噬菌体山东1的全基因组序列分析

Complete genome sequence analysis of the novel mycobacteriophage Shandong1.

作者信息

Fan Xiangyu, Gao Xiaomeng, Wang Quanbin, Shen Junyu, Zhou Lei, Xie Jianping

机构信息

School of Biological Science and Technology, University of Jinan, Shandong, 250022, China.

Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environment of Three Gorges Reservoir, Ministry of Education, School of Life Sciences, Southwest University, Chongqing, 400715, China.

出版信息

Arch Virol. 2017 Dec;162(12):3903-3905. doi: 10.1007/s00705-017-3534-7. Epub 2017 Aug 21.

DOI:10.1007/s00705-017-3534-7

PMID:28828700

Abstract

In this study, we isolated a mycobacteriophage infecting Mycobacterium smegmatis mc155 from a soil sample collected in Shandong Province in China. This phage was named Shandong1. It is a member of the family Siphoviridae with an isometric head and a long tail. Its genome was found to be 60,618 bp long with 67.46% G + C content and 96 putative protein-coding genes. No tRNA-encoding genes were identified. Comparative genomics analysis showed that the mycobacteriophage Shandong1 should be considered a member of a new species in mycobacteriophage cluster K.

摘要

在本研究中，我们从中国山东省采集的一份土壤样本中分离出一种感染耻垢分枝杆菌mc155的分枝杆菌噬菌体。该噬菌体被命名为山东1。它是长尾噬菌体科的成员，具有等轴状头部和一条长尾。其基因组长度为60,618 bp，G + C含量为67.46%，有96个推定的蛋白质编码基因。未鉴定出编码tRNA的基因。比较基因组学分析表明，分枝杆菌噬菌体山东1应被视为分枝杆菌噬菌体簇K中一个新物种的成员。