从中国土壤中分离出的分枝杆菌噬菌体SWU1的基因组和蛋白质组特征
Genomic and proteomic features of mycobacteriophage SWU1 isolated from China soil.
作者信息
Fan Xiangyu, Yan Jianlong, Xie Longxiang, Zeng Lanying, Young Ryland F, Xie Jianping
机构信息
Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environment of Three Gorges Reservoir, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China; School of Biological Science and Technology, University of Jinan, Shandong 250022, China.
Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environment of Three Gorges Reservoir, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China.
出版信息
Gene. 2015 Apr 25;561(1):45-53. doi: 10.1016/j.gene.2015.02.053. Epub 2015 Feb 19.
Mycobacteriophage SWU1 is a newly isolated phage from soil sample collected in Sichuan province, China using Mycobacterium smegmatis mc(2)155 as host. Plaque, phage morphology and one-step growth curve were characterized. The complete genomic sequence of phage SWU1 was determined by shotgun sequencing. The ends of SWU1 were determined. Structural proteins of SWU1 were analyzed by NanoLC-ESI-MS/MS. Seven ORFs were identified as structural protein encoded by SWU1 genome. The genetic basis underlying the SWU1 plaque was explored using comparative genomics. Prophages homologous to SWU1 were identified in two pathogens, Segniliparus rugosus ATCC BAA-974 and Mycobacterium rhodesiae JS60. Genus Segniliparus is a member of the order Corynebacteriales. To our knowledge, this is the first report of Mycobacterium prophages in different genera.
分枝杆菌噬菌体SWU1是从中国四川省采集的土壤样本中新分离出的一种噬菌体，它以耻垢分枝杆菌mc(2)155作为宿主。对噬菌斑、噬菌体形态和一步生长曲线进行了表征。通过鸟枪法测序确定了噬菌体SWU1的完整基因组序列。确定了SWU1的末端。通过纳升液相色谱-电喷雾串联质谱法（NanoLC-ESI-MS/MS）分析了SWU1的结构蛋白。七个开放阅读框被鉴定为SWU1基因组编码的结构蛋白。利用比较基因组学探索了SWU1噬菌斑的遗传基础。在两种病原体，即皱纹栖热放线菌ATCC BAA-974和罗得西亚分枝杆菌JS60中鉴定出了与SWU1同源的原噬菌体。栖热放线菌属是棒杆菌目的一个成员。据我们所知，这是不同属中结核分枝杆菌原噬菌体的首次报道。
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