Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing Municipal Key Laboratory of Karst Environment, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China.
Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing Municipal Key Laboratory of Karst Environment, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China; School of Biological Science and Technology, University of Jinan, Jinan 250022, China.
Infect Genet Evol. 2021 Jan;87:104665. doi: 10.1016/j.meegid.2020.104665. Epub 2020 Dec 3.
Phage therapy, especially combination with antibiotics, was revitalized to control the antibiotics resistance. Mycobacteriophage, the phage of mycobacterium with the most notorious Mycobacterium tuberculosis (M. tuberculosis), was intensively explored. A novel mycobacteriophage SWU2 was isolated from a soil sample collected at Nanchang city, Jiangxi province, China, by using Mycolicibacterium smegmatis (M. smegmatis) mc 155 as the host. Phage morphology and biology were characterized. Phage structure proteins were analyzed by LC-MS/MS. The putative functions of phage proteins and multi-genome comparison were performed with bioinformatics. The transmission electron microscopy result indicated that this phage belongs to Siphoviridae of Caudovirales. Plaques of SWU2 appeared clear but small. In a one-step growth test, we demonstrated that SWU2 had a latent period of 30 min and a logarithmic phase of 120 min. Among the 76 predicted Open Reading Frames (ORFs), 9 ORFs were identified as phage structure proteins of SWU2. The assembled phage genome size is 50,013 bp, with 62.7% of G + C content. SWU2 genome sequence shares 88% identity with Mycobacterium phages HINdeR and Timshel, differing in substitutions, insertions and deletions in SWU2. Phylogenetic tree revealed that SWU2 is grouped into A7 sub-cluster. There are several substitutions, insertions and deletions in SWU2 genome in comparison with close cousin phages HINdeR and Timshel. The new phage adds another dimension of abundance to the mycobacteriophages.
噬菌体疗法，尤其是与抗生素联合使用，已被重新用于控制抗生素耐药性。分枝杆菌噬菌体是分枝杆菌的噬菌体，其中最臭名昭著的是结核分枝杆菌（M. tuberculosis），目前正在进行深入研究。从中国江西省南昌市采集的土壤样本中，使用耻垢分枝杆菌（M. smegmatis）mc 155 作为宿主，分离到一种新型分枝杆菌噬菌体 SWU2。对噬菌体的形态和生物学特性进行了研究。通过 LC-MS/MS 分析了噬菌体结构蛋白。利用生物信息学方法对噬菌体蛋白的假定功能和多基因组比较进行了分析。透射电子显微镜结果表明，该噬菌体属于有尾病毒目的长尾病毒科。SWU2 的噬菌斑清晰但较小。在一步生长试验中，我们证明 SWU2 的潜伏期为 30 分钟，对数期为 120 分钟。在预测的 76 个开放阅读框（ORFs）中，有 9 个 ORFs 被鉴定为 SWU2 的噬菌体结构蛋白。组装的噬菌体基因组大小为 50,013 bp，G+C 含量为 62.7%。SWU2 基因组序列与分枝杆菌噬菌体 HINdeR 和 Timshel 的同源性为 88%，在 SWU2 中存在取代、插入和缺失。系统发育树显示，SWU2 被分为 A7 亚群。与近亲噬菌体 HINdeR 和 Timshel 相比，SWU2 基因组中存在几个取代、插入和缺失。新噬菌体增加了分枝杆菌噬菌体的丰富度。
