Ahn Jong Hoon, Mo Eun Jin, Jo Yang Hee, Kim Seon Beom, Hwang Bang Yeon, Lee Mi Kyeong
a College of Pharmacy , Chungbuk National University , Cheongju , Korea.
Biosci Biotechnol Biochem. 2017 Oct;81(10):1973-1977. doi: 10.1080/09168451.2017.1361807. Epub 2017 Aug 22.
Efficient preparation of loganin from Cornus officinalis fruits was investigated. First, effect of extraction conditions on loganin yield was measured. The loganin content in C. officinalis extract was greatly affected by ethanol concentration and extraction time whereas extraction temperature exerted relatively little effect. Response surface methodology with Box-Behnken design suggested optimized extraction condition for maximum loganin yield as ethanol concentration, 32.0%; temperature 46.2 °C and extraction time, 46.7 min, which yielded 10.4 μg loganin/mg dried fruit. Next, the effect of maturation stage of C. officinalis fruits on loganin content was investigated. The loganin content in the extract of C. officinalis fruits was decreased as the maturation process. The loganin content in the unripe fruits was 18.0 μg/mg extract whereas reduced to 13.3 μg/mg extract for ripe fruits. Taken together, our present study suggested the importance of extraction condition and maturation stages for efficient preparation of loganin from C. officinalis fruits.
研究了从山茱萸果实中高效制备马钱苷的方法。首先，测定了提取条件对马钱苷产量的影响。山茱萸提取物中的马钱苷含量受乙醇浓度和提取时间的影响很大，而提取温度的影响相对较小。采用Box-Behnken设计的响应面法得出，以乙醇浓度32.0%、温度46.2℃、提取时间46.7分钟为优化提取条件时，马钱苷产量最高，每毫克干果可产出10.4μg马钱苷。接下来，研究了山茱萸果实成熟阶段对马钱苷含量的影响。随着成熟过程，山茱萸果实提取物中的马钱苷含量降低。未成熟果实提取物中的马钱苷含量为18.0μg/mg，而成熟果实提取物中的马钱苷含量降至13.3μg/mg。综上所述，我们目前的研究表明，提取条件和成熟阶段对于从山茱萸果实中高效制备马钱苷具有重要意义。
