College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 151-742, Republic of Korea.
Phytomedicine. 2012 Feb 15;19(3-4):317-21. doi: 10.1016/j.phymed.2011.08.068. Epub 2011 Oct 5.
The methanolic extract of the fruits of Cornus officinalis S et Z. (Cornaceae) showed the significant neuroprotective activity against glutamate-induced toxicity in HT22 hippocampal cells. Chemical profile of n-BuOH fraction of the methanolic extract of C. officinalis fruits, which showed the most potent activity, was established using HPLC-diode array detector-electrospray-MS (HPLC-DAD-ESI-MS). Through bioactivity-guided isolation, five iridoid glycosides including one new compound, 7-O-butylmorroniside (1), loganin (2), morroniside (3), 7R-O-methylmorroniside (4), 7S-O-methylmorroniside (5) were isolated from the n-BuOH fraction. The protective activities of the isolated compounds, themselves, were not statistically significant. However, the hydrolyzed products of compounds 1, 4 and 5 significantly protected glutamate-injured HT22 cells up to 78±2.2%, 60±3.2% and 59±2.5% of non-treated control, respectively.
山茱萸（山茱萸科）果实的甲醇提取物对 HT22 海马细胞中谷氨酸诱导的毒性表现出显著的神经保护活性。显示出最强活性的山茱萸果实甲醇提取物的正丁醇部分的化学特征通过高效液相色谱-二极管阵列检测器-电喷雾质谱（HPLC-DAD-ESI-MS）建立。通过生物活性导向分离，从正丁醇部分分离得到了五种环烯醚萜苷，包括一种新化合物 7-O-丁基莫罗尼苷（1）、马钱苷（2）、莫罗尼苷（3）、7R-O-甲基莫罗尼苷（4）和 7S-O-甲基莫罗尼苷（5）。分离得到的化合物本身的保护活性没有统计学意义。然而，化合物 1、4 和 5 的水解产物对谷氨酸损伤的 HT22 细胞的保护作用分别高达非处理对照的 78±2.2%、60±3.2%和 59±2.5%。
