Wasoontharawat Metinee, Phongsopitanun Wongsakorn, Siriudom Sakutala, Tanasupawat Somboon
Department of Biology, Faculty of Science, Udon Thani Rajabhat University, Udon Thani 41000, Thailand.
Department of Biology, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, Thailand.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3226-3230. doi: 10.1099/ijsem.0.002088. Epub 2017 Aug 22.
A Gram-stain-positive, rod-shaped and endospore-forming bacterium, strain RC11T, isolated from ant nest soil collected from Udon Thani Province, Thailand was characterized in a taxonomic study based on a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain RC11T was affiliated to the genus Paenibacillus and was closely related to Paenibacillus phyllosphaerae LMG 22192T with 98.1 % sequence similarity. Strain RC11T contained meso-diaminopimelic in the cell-wall peptidoglycan. The major menaquinone was MK-7. Anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and C16 : 0 were the predominant cellular fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids. The DNA G+C content was 57.9 mol%. In addition, strain RC11T and P. phyllosphaerae LMG 22192T showed a low level of DNA-DNA relatedness (15.6-33.2 %). Therefore, strain RC11T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus aurantiacus sp. nov. is proposed. The type strain is RC11T (=KCTC 33816T=LMG 29659T=TISTR 2452T).
从泰国乌隆他尼府采集的蚁巢土壤中分离出的一株革兰氏阳性、杆状且形成芽孢的细菌RC11T，在一项基于多相分类法的分类学研究中进行了特征描述。基于16S rRNA基因序列分析，菌株RC11T隶属于芽孢杆菌属，与叶鞘芽孢杆菌Paenibacillus phyllosphaerae LMG 22192T密切相关，序列相似性为98.1%。菌株RC11T的细胞壁肽聚糖中含有内消旋二氨基庚二酸。主要的甲基萘醌是MK-7。anteiso-C15 : 0、iso-C16 : 0、anteiso-C17 : 0和C16 : 0是主要的细胞脂肪酸。二磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油是主要的极性脂质。DNA的G+C含量为57.9 mol%。此外，菌株RC11T与叶鞘芽孢杆菌P. phyllosphaerae LMG 22192T显示出较低水平的DNA-DNA相关性（15.6-33.2%）。因此，菌株RC11T代表芽孢杆菌属的一个新物种，为此提出名为橙色芽孢杆菌Paenibacillus aurantiacus sp. nov.。模式菌株为RC11T（=KCTC 33816T=LMG 29659T=TISTR 2452T）。
