Shahraki Abdolrazagh Hashemi, Çavuşoğlu Cengiz, Borroni Emanuele, Heidarieh Parvin, Koksalan Orhan Kaya, Cabibbe Andrea Maurizio, Hashemzadeh Mohamad, Mariottini Alessandro, Mostafavi Ehsan, Cittaro Davide, Feizabadi Mohamad Mehdi, Lazarevic Dejan, Yaghmaei Farhad, Molinari Gian Lorenzo, Camaggi Anna, Tortoli Enrico
Department of Epidemiology, Pasteur Institute of Iran, Tehran, Iran.
Department of Medical Microbiology, Ege University, Izmir, Turkey.
Int J Syst Evol Microbiol. 2015 Feb;65(Pt 2):510-515. doi: 10.1099/ijs.0.064832-0. Epub 2014 Nov 11.
Six strains of a rapidly growing scotochromogenic mycobacterium were isolated from pulmonary specimens of independent patients. Biochemical and cultural tests were not suitable for their identification. The mycolic acid pattern analysed by HPLC was different from that of any other mycobacterium. Genotypic characterization, targeting seven housekeeping genes, revealed the presence of microheterogeneity in all of them. Different species were more closely related to the test strains in various regions: the type strain of Mycobacterium moriokaense showed 99.0 % 16S rRNA gene sequence similarity, and 91.5-96.5 % similarity for the remaining six regions. The whole genome sequences of the proposed type strain and that of M. moriokaense presented an average nucleotide identity (ANI) of 82.9 %. Phylogenetic analysis produced poorly robust trees in most genes with the exception of rpoB and sodA where Mycobacterium flavescens and Mycobacterium novocastrense were the closest species. This phylogenetic relatedness was confirmed by the tree inferred from five concatenated genes, which was very robust. The polyphasic characterization of the test strains, supported by the ANI value, demonstrates that they belong to a previously unreported species, for which the name Mycobacterium celeriflavum sp. nov. is proposed. The type strain is AFPC-000207(T) ( = DSM 46765(T) = JCM 18439(T)).
从不同患者的肺部标本中分离出6株快速生长的暗产色分枝杆菌。生化和培养试验不适用于它们的鉴定。通过高效液相色谱分析的分枝菌酸模式与其他任何分枝杆菌的模式不同。针对7个管家基因的基因分型显示，所有菌株均存在微异质性。不同物种在不同区域与测试菌株的亲缘关系更为密切：盛冈分枝杆菌模式菌株的16S rRNA基因序列相似度为99.0%，其余6个区域的相似度为91.5 - 96.5%。拟模式菌株和盛冈分枝杆菌的全基因组序列的平均核苷酸同一性（ANI）为82.9%。系统发育分析在大多数基因中产生的树状图稳健性较差，但rpoB和sodA基因除外，在这两个基因中，微黄分枝杆菌和新卡斯特里分枝杆菌是最接近的物种。从5个串联基因推断出的树状图证实了这种系统发育相关性，该树状图非常稳健。测试菌株的多相特征，得到ANI值的支持，表明它们属于一个以前未报道的物种，为此提出了新名称微黄分枝杆菌（Mycobacterium celeriflavum sp. nov.）。模式菌株为AFPC - 000207(T)（= DSM 46765(T)= JCM 18439(T)）。
