van der Korput J A, Hilkens J, Kroezen V, Zwarthoff E C, Trapman J
J Gen Virol. 1985 Mar;66 ( Pt 3):493-502. doi: 10.1099/0022-1317-66-3-493.
In order to determine the chromosomal localization of the murine interferon-alpha (MuIFN-alpha) and murine interferon-beta (MuIFN-beta) genes the DNAs of a panel of somatic cell hybrids were analysed by Southern blot hybridization. The hybrid cells were derived from E36 Chinese hamster cells and GRSL or GR MaTu mouse cells and retained all hamster chromosomes but segregated mouse chromosomes. The MuIFN-alpha probe used was a 0.7 kb HindIII-EcoRI fragment derived from the MuIFN-alpha 1 gene which hybridized with both mouse and hamster DNA. However, four fragments present in EcoRI digests of mouse DNA were clearly absent from the hybridization profile of EcoRI-digested hamster DNA and could be used for detection of MuIFN-alpha sequences in the hybrid cells. The MuIFN-beta probe, a 0.5 kb BglII-BamHI fragment derived from the MuIFN-beta gene, hybridized with a 2.6 kb EcoRI fragment of mouse DNA and only weakly cross-hybridized with a 4.8 kb EcoRI fragment in hamster DNA. Southern blot analysis of DNA from mouse/hamster hybrids compared with the analysis of chromosome markers showed that both the MuIFN-alpha and the MuIFN-beta genes are located on chromosome 4. Analysis of DNA from hybrids that contained only part of chromosome 4 indicated that the MuIFN-alpha gene family and the MuIFN-beta gene are situated at the centromere-proximal region of the chromosome.
为了确定小鼠α干扰素（MuIFN-α）和小鼠β干扰素（MuIFN-β）基因的染色体定位，通过Southern印迹杂交分析了一组体细胞杂种的DNA。杂种细胞来源于E36中国仓鼠细胞和GRSL或GR MaTu小鼠细胞，保留了所有仓鼠染色体，但小鼠染色体发生了分离。所用的MuIFN-α探针是一个0.7 kb的HindIII-EcoRI片段，来源于MuIFN-α1基因，它能与小鼠和仓鼠的DNA杂交。然而，在小鼠DNA的EcoRI酶切片段中存在的四个片段在仓鼠DNA的EcoRI酶切杂交图谱中明显缺失，可用于检测杂种细胞中的MuIFN-α序列。MuIFN-β探针是一个0.5 kb的BglII-BamHI片段，来源于MuIFN-β基因，它与小鼠DNA的一个2.6 kb的EcoRI片段杂交，与仓鼠DNA中一个4.8 kb的EcoRI片段仅有微弱的交叉杂交。与染色体标记分析相比，对小鼠/仓鼠杂种DNA的Southern印迹分析表明，MuIFN-α和MuIFN-β基因都位于4号染色体上。对仅含有4号染色体部分片段的杂种DNA分析表明，MuIFN-α基因家族和MuIFN-β基因位于该染色体着丝粒近端区域。
