小鼠免疫干扰素（IFN-γ）基因位于第10号染色体上。

Mouse immune interferon (IFN-gamma) gene is on chromosome 10.

作者信息

Naylor S L, Gray P W, Lalley P A

出版信息

Somat Cell Mol Genet. 1984 Sep;10(5):531-4. doi: 10.1007/BF01534857.

Abstract

A cDNA clone for mouse immune interferon has been used to map the mouse interferon gamma gene (Ifg) to a specific chromosome. This clone, which contains a 638-bp insert detects an 18-kb HindIII fragment of mouse DNA. The presence of the mouse Ifg gene in cel hybrids and its chromosomal location were determined by assaying cell hybrid DNA for the presence of the 18-kb HindIII fragment by Southern filter hybridization. Under the hybridization conditions used, Chinese hamster DNA did not hybridize to the cDNA probe. The segregation of mouse chromosomes in cell hybrids indicated that Ifg is located on chromosome 10. Previously, we have mapped immune interferon to the p12.05----qter region of chromosome 12 in humans (1). This region of chromosome 12 also contains the genes for peptidase B and citrate synthase. The homologous genes in mouse are also located on chromosome 10, suggesting that these genes comprise a conserved linkage group.

摘要

一个用于小鼠免疫干扰素的cDNA克隆已被用于将小鼠干扰素γ基因（Ifg）定位到特定染色体上。这个包含638个碱基对插入片段的克隆检测到小鼠DNA的一个18kb HindIII片段。通过Southern滤膜杂交检测细胞杂交DNA中18kb HindIII片段的存在，确定了细胞杂种中小鼠Ifg基因的存在及其染色体定位。在所使用的杂交条件下，中国仓鼠DNA不与cDNA探针杂交。细胞杂种中小鼠染色体的分离表明Ifg位于第10号染色体上。此前，我们已将人类免疫干扰素定位到第12号染色体的p12.05----qter区域（1）。第12号染色体的这个区域还包含肽酶B和柠檬酸合酶的基因。小鼠中的同源基因也位于第10号染色体上，这表明这些基因构成一个保守的连锁群。