Naylor S L, Sakaguchi A Y, Szoka P, Hendy G N, Kronenberg H M, Rich A, Shows T B
Somatic Cell Genet. 1983 Sep;9(5):609-16. doi: 10.1007/BF01574261.
The human gene for parathyroid hormone (PTH) was chromosomally mapped using human-rodent hybrids and Southern filter hybridization of cell hybrid DNA. A recombinant DNA probe containing human PTH cDNA insert (pPTHm122) hybridized to a 3.7-kb fragment in human DNA cleaved with the restriction enzyme EcoRI. By correlating the presence of this fragment in somatic cell hybrid DNA with the human chromosomal content of the hybrid cells, the PTH gene was mapped to the short arm of the chromosome 11.
利用人-啮齿动物杂种细胞及细胞杂种DNA的Southern印迹杂交技术,对甲状旁腺激素(PTH)的人类基因进行了染色体定位。一个含有人类PTH cDNA插入片段(pPTHm122)的重组DNA探针,与经限制性内切酶EcoRI切割的人类DNA中的一个3.7kb片段杂交。通过将该片段在体细胞杂种DNA中的存在情况与杂种细胞的人类染色体组成相关联,将PTH基因定位到了11号染色体的短臂上。