Kim Tae M, Park Hyojun, Lee Kyo W, Choi Eun W, Moon Sang H, Lee Yong S, Cho Kahee, Park Wan J, Park Jae B, Kim Sung J
Laboratory Animal Research Center, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Seoul, Korea.
Department of Surgery, Division of Transplantation, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Comp Med. 2017 Aug 1;67(4):356-359.
Our investigation of indoor-housed cynomolgus macaques (Macaca fascicularis) by using automated identification followed by antibiotic susceptibility testing revealed 1 of 7 immunocompetent animals and 2 of 9 immunosuppressed monkeys as carriers of methicillin-resistant Staphylococcus aureus (MRSA). Follow-up management involving mupirocin treatment resulted in the conversion of the 3 MRSA carriers into MRSA-negative cases. Prospective assessment of newly imported monkeys involving 24-h culture of nasal swabs on chromogenic agar revealed that 22% (18 of 82 animals) were MRSA-positive. Mupirocin treatment successfully converted all of the MRSA-positive macaques into non-carriers, suggesting the feasibility of this simple, one-step screening procedure for rapidly identifying MRSA carriers in large cohorts. In addition, 8 animals that had been diagnosed MRSA-positive and subsequently treated with mupirocin demonstrated no recolonization during follow-up, even under immunosuppressive conditions. We propose rapid screening using chromogenic agar followed by mupirocin treatment as a time- and cost-effective regimen for managing MRSA in cynomolgus monkeys.
我们通过自动识别随后进行抗生素敏感性测试，对室内饲养的食蟹猴（猕猴）进行调查，结果显示，7只免疫功能正常的动物中有1只、9只免疫抑制的猴子中有2只为耐甲氧西林金黄色葡萄球菌（MRSA）携带者。采用莫匹罗星治疗的后续管理使3例MRSA携带者转变为MRSA阴性病例。对新引进猴子进行的前瞻性评估，包括在显色琼脂上对鼻拭子进行24小时培养，结果显示22%（82只动物中有18只）为MRSA阳性。莫匹罗星治疗成功地使所有MRSA阳性猕猴转变为非携带者，这表明这种简单的一步筛查程序在大群体中快速识别MRSA携带者是可行的。此外，8只被诊断为MRSA阳性并随后接受莫匹罗星治疗的动物在随访期间未出现重新定植，即使在免疫抑制条件下也是如此。我们建议使用显色琼脂进行快速筛查，随后进行莫匹罗星治疗，作为一种经济高效的方案来管理食蟹猴中的MRSA。
