• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

绵羊中粗制幼虫蛋白和重组体细胞蛋白26/23（rHcp26/23）免疫接种的评估。

Evaluation of crude larval protein and recombinant somatic protein 26/23 (rHcp26/23) immunization against in sheep.

作者信息

Kandil Omnia M, Abdelrahman Khaled A, Shalaby Hatem A, Hendawy Seham H M, El Ezz Nadia M T Abu, Nassar Somia A, Miller James E

机构信息

Department of Parasitology and Animal Diseases, National Research Centre, Dokki, P.O. Box 12622, Giza, Egypt.

Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.

出版信息

Vet World. 2017 Jul;10(7):758-763. doi: 10.14202/vetworld.2017.758-763. Epub 2017 Jul 8.

DOI:10.14202/vetworld.2017.758-763
PMID:28831218
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5553143/
Abstract

AIM

The aim of this study was to evaluate the potential possibility of crude larval and recombinant (rHcp26/23) antigens of for immunization to control sheep hemonchosis.

MATERIALS AND METHODS

A total of 21 lambs were divided into five groups. Lambs were immunized with larval and recombinant (rHcp26/23) proteins at day 0 and day 14 and after that challenged with 5000 infective larvae of on day 42. An unvaccinated positive control group was challenged with L3 in the meantime. An unvaccinated negative control group was not challenged.

RESULTS

Fecal egg count reduction taking after challenge for rHcp26/23 and larval antigens was 92.2% and 38.2%, respectively, compared with the positive control group. Vaccine incited protection in rHcp26/23 and larval immunization was reflected in significant (p<0.05) decreases in worm burden; 59.9% and 40.1%, respectively.

CONCLUSION

Recombinant rHcp26/23 vaccine induced a partial immune response and had immune-protective effect against sheep hemonchosis.

摘要

目的

本研究旨在评估用幼虫粗抗原和重组（rHcp26/23）抗原免疫以控制绵羊血矛线虫病的潜在可能性。

材料与方法

将21只羔羊分为五组。在第0天和第14天用幼虫和重组（rHcp26/23）蛋白对羔羊进行免疫，之后在第42天用5000条 感染性幼虫进行攻虫。同时，一个未接种疫苗的阳性对照组用L3进行攻虫。一个未接种疫苗的阴性对照组不进行攻虫。

结果

与阳性对照组相比，rHcp26/23和幼虫抗原攻虫后的粪便虫卵计数减少率分别为92.2%和38.2%。rHcp26/23和幼虫免疫中疫苗诱导的保护作用表现为虫体负荷显著（p<0.05）降低，分别为59.9%和40.1%。

结论

重组rHcp26/23疫苗诱导了部分免疫反应，对绵羊血矛线虫病具有免疫保护作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de50/5553143/d7f8dcc6e14f/VetWorld-10-758-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de50/5553143/56ec6908ba08/VetWorld-10-758-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de50/5553143/4fb6075499c6/VetWorld-10-758-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de50/5553143/93e6e16e4b1d/VetWorld-10-758-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de50/5553143/99c4aacb2368/VetWorld-10-758-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de50/5553143/d7f8dcc6e14f/VetWorld-10-758-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de50/5553143/56ec6908ba08/VetWorld-10-758-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de50/5553143/4fb6075499c6/VetWorld-10-758-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de50/5553143/93e6e16e4b1d/VetWorld-10-758-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de50/5553143/99c4aacb2368/VetWorld-10-758-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de50/5553143/d7f8dcc6e14f/VetWorld-10-758-g005.jpg

相似文献

1
Evaluation of crude larval protein and recombinant somatic protein 26/23 (rHcp26/23) immunization against in sheep.绵羊中粗制幼虫蛋白和重组体细胞蛋白26/23（rHcp26/23）免疫接种的评估。
Vet World. 2017 Jul;10(7):758-763. doi: 10.14202/vetworld.2017.758-763. Epub 2017 Jul 8.
2
Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp26/23).用捻转血矛线虫重组体壁抗原（rHcp26/23）对羔羊血矛线虫病进行免疫接种
Vet Med Int. 2010;2010:852146. doi: 10.4061/2010/852146. Epub 2010 Jun 29.
3
Vaccination of goats with a glutathione peroxidase DNA vaccine induced partial protection against Haemonchus contortus infection.给山羊接种谷胱甘肽过氧化物酶 DNA 疫苗可诱导部分抵抗捻转血矛线虫感染的保护作用。
Vet Parasitol. 2011 Dec 15;182(2-4):239-47. doi: 10.1016/j.vetpar.2011.05.024. Epub 2011 May 27.
4
Antibodies to surface epitopes of the carbohydrate larval antigen CarLA are associated with passive protection in strongylid nematode challenge infections.针对碳水化合物幼虫抗原CarLA表面表位的抗体与类圆线虫攻击感染中的被动保护作用相关。
Parasite Immunol. 2008 Nov-Dec;30(11-12):577-84. doi: 10.1111/j.1365-3024.2008.01058.x.
5
Vaccination of goats with DNA vaccine encoding Dim-1 induced partial protection against Haemonchus contortus: a preliminary experimental study.用编码 Dim-1 的 DNA 疫苗给山羊接种可诱导对捻转血矛线虫的部分保护：初步实验研究。
Res Vet Sci. 2013 Aug;95(1):189-99. doi: 10.1016/j.rvsc.2013.02.020. Epub 2013 Mar 29.
6
Immune response to Haemonchus contortus and Haemonchus placei in sheep and its role on parasite specificity.绵羊对捻转血矛线虫和蛇形毛圆线虫的免疫反应及其对寄生虫特异性的作用。
Vet Parasitol. 2014 Jun 16;203(1-2):127-38. doi: 10.1016/j.vetpar.2014.02.048. Epub 2014 Mar 2.
7
Can Haemonchus placei-primary infected naïve lambs withstand Haemonchus contortus infections?感染了原虫的初产羔羊能否抵御捻转血矛线虫感染？
Res Vet Sci. 2017 Oct;114:136-142. doi: 10.1016/j.rvsc.2017.02.017. Epub 2017 Feb 28.
8
Vaccination of goats with glyceraldehyde-3-phosphate dehydrogenase DNA vaccine induced partial protection against Haemonchus contortus.用甘油醛-3-磷酸脱氢酶DNA疫苗对山羊进行接种可诱导对捻转血矛线虫产生部分保护作用。
Vet Immunol Immunopathol. 2012 Oct 15;149(3-4):177-85. doi: 10.1016/j.vetimm.2012.06.016. Epub 2012 Jun 19.
9
Immunization with recombinant rHc23 partially protects lambs against trickle infections by Haemonchus contortus.用重组 rHc23 免疫可部分保护羔羊免受捻转血矛线虫的滴注感染。
BMC Vet Res. 2019 Sep 18;15(1):333. doi: 10.1186/s12917-019-2084-5.
10
DNA vaccine encoding Haemonchus contortus actin induces partial protection in goats.编码捻转血矛线虫肌动蛋白的DNA疫苗可诱导山羊产生部分保护作用。
Acta Parasitol. 2014 Oct;59(4):698-709. doi: 10.2478/s11686-014-0298-z. Epub 2014 Sep 20.

引用本文的文献

1
Immunity to gastrointestinal nematodes in ruminants: effector cell mechanisms and cytokines.反刍动物对胃肠道线虫的免疫：效应细胞机制与细胞因子
J Parasit Dis. 2018 Dec;42(4):471-482. doi: 10.1007/s12639-018-1023-x. Epub 2018 Aug 9.
2
Vaccination of lambs against Haemonchus contortus with the recombinant rHc23. Effect of adjuvant and antigen dose.羔羊用重组 rHc23 针对捻转血矛线虫的免疫接种。佐剂和抗原剂量的影响。
PLoS One. 2018 Mar 7;13(3):e0193118. doi: 10.1371/journal.pone.0193118. eCollection 2018.

本文引用的文献

1
Evaluation of different antigens for diagnosis of sheep haemonchosis by ELISA and their cross reactivity with other helminthes.通过酶联免疫吸附测定法（ELISA）评估用于诊断绵羊血矛线虫病的不同抗原及其与其他蠕虫的交叉反应性。
J Parasit Dis. 2017 Sep;41(3):678-683. doi: 10.1007/s12639-016-0865-3. Epub 2016 Nov 23.
2
Understanding Haemonchus contortus Better Through Genomics and Transcriptomics.通过基因组学和转录组学更好地了解捻转血矛线虫
Adv Parasitol. 2016;93:519-67. doi: 10.1016/bs.apar.2016.02.015. Epub 2016 Apr 5.
3
Vaccination of lambs with the recombinant protein rHc23 elicits significant protection against Haemonchus contortus challenge.
用重组蛋白rHc23对羔羊进行疫苗接种可引发对捻转血矛线虫攻击的显著保护作用。
Vet Parasitol. 2015 Jun 30;211(1-2):54-9. doi: 10.1016/j.vetpar.2015.04.029. Epub 2015 May 8.
4
Epidemiology, sero-diagnosis and therapeutic studies on nematodes infection in balochi range-sheep at district quetta, balochistan, pakistan.巴基斯坦俾路支省奎达地区俾路支山地绵羊线虫感染的流行病学、血清学诊断及治疗研究
Iran J Parasitol. 2014 Apr-Jun;9(2):169-80.
5
Immunoprotection in sheep against Haemonchus contortus using its thiol-purified excretory/secretory proteins.利用绵羊捻转血矛线虫硫醇纯化的排泄/分泌蛋白对绵羊进行免疫保护。
Vet Res Forum. 2012 Fall;3(4):239-44.
6
Attempts to vaccinate ewes and their lambs against natural infection with Haemonchus contortus in a tropical environment.在热带环境中尝试给母羊及其羔羊接种疫苗以抵抗捻转血矛线虫的自然感染。
Int J Parasitol. 2014 Dec;44(14):1049-54. doi: 10.1016/j.ijpara.2014.07.007. Epub 2014 Aug 18.
7
Vaccination of lambs against Haemonchus contortus infection with a somatic protein (Hc23) from adult helminths.用来自成年蠕虫的一种体蛋白（Hc23）对羔羊进行接种，以预防捻转血矛线虫感染。
Int J Parasitol. 2014 Jun;44(7):429-36. doi: 10.1016/j.ijpara.2014.02.009. Epub 2014 Apr 20.
8
Novel expression of Haemonchus contortus vaccine candidate aminopeptidase H11 using the free-living nematode Caenorhabditis elegans.利用自由生活的线虫秀丽隐杆线虫表达捻转血矛线虫疫苗候选物氨肽酶 H11 的新方法。
Vet Res. 2013 Dec 1;44(1):111. doi: 10.1186/1297-9716-44-111.
9
The effect of different adjuvants on immune parameters and protection following vaccination of sheep with a larval-specific antigen of the gastrointestinal nematode, Haemonchus contortus.用胃肠道线虫捻转血矛线虫的幼虫特异性抗原对绵羊进行疫苗接种后，不同佐剂对免疫参数和保护作用的影响。
PLoS One. 2013 Oct 21;8(10):e78357. doi: 10.1371/journal.pone.0078357. eCollection 2013.
10
Field vaccination of sheep with a larval-specific antigen of the gastrointestinal nematode, Haemonchus contortus, confers significant protection against an experimental challenge infection.用胃肠道线虫捻转血矛线虫的幼虫特异性抗原对绵羊进行现场接种可显著预防实验性攻毒感染。
Vaccine. 2012 Nov 26;30(50):7199-204. doi: 10.1016/j.vaccine.2012.10.019. Epub 2012 Oct 27.