绵羊中粗制幼虫蛋白和重组体细胞蛋白26/23（rHcp26/23）免疫接种的评估。
Evaluation of crude larval protein and recombinant somatic protein 26/23 (rHcp26/23) immunization against in sheep.
作者信息
Kandil Omnia M, Abdelrahman Khaled A, Shalaby Hatem A, Hendawy Seham H M, El Ezz Nadia M T Abu, Nassar Somia A, Miller James E
机构信息
Department of Parasitology and Animal Diseases, National Research Centre, Dokki, P.O. Box 12622, Giza, Egypt.
Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
出版信息
Vet World. 2017 Jul;10(7):758-763. doi: 10.14202/vetworld.2017.758-763. Epub 2017 Jul 8.
AIM
The aim of this study was to evaluate the potential possibility of crude larval and recombinant (rHcp26/23) antigens of for immunization to control sheep hemonchosis.
MATERIALS AND METHODS
A total of 21 lambs were divided into five groups. Lambs were immunized with larval and recombinant (rHcp26/23) proteins at day 0 and day 14 and after that challenged with 5000 infective larvae of on day 42. An unvaccinated positive control group was challenged with L3 in the meantime. An unvaccinated negative control group was not challenged.
RESULTS
Fecal egg count reduction taking after challenge for rHcp26/23 and larval antigens was 92.2% and 38.2%, respectively, compared with the positive control group. Vaccine incited protection in rHcp26/23 and larval immunization was reflected in significant (p<0.05) decreases in worm burden; 59.9% and 40.1%, respectively.
CONCLUSION
Recombinant rHcp26/23 vaccine induced a partial immune response and had immune-protective effect against sheep hemonchosis.
目的
本研究旨在评估用幼虫粗抗原和重组（rHcp26/23）抗原免疫以控制绵羊血矛线虫病的潜在可能性。
材料与方法
将21只羔羊分为五组。在第0天和第14天用幼虫和重组（rHcp26/23）蛋白对羔羊进行免疫，之后在第42天用5000条 感染性幼虫进行攻虫。同时，一个未接种疫苗的阳性对照组用L3进行攻虫。一个未接种疫苗的阴性对照组不进行攻虫。
结果
与阳性对照组相比，rHcp26/23和幼虫抗原攻虫后的粪便虫卵计数减少率分别为92.2%和38.2%。rHcp26/23和幼虫免疫中疫苗诱导的保护作用表现为虫体负荷显著（p<0.05）降低，分别为59.9%和40.1%。
结论
重组rHcp26/23疫苗诱导了部分免疫反应，对绵羊血矛线虫病具有免疫保护作用。
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