Piedrafita David, Preston Sarah, Kemp Joanna, de Veer Michael, Sherrard Jayne, Kraska Troy, Elhay Martin, Meeusen Els
School of Biomedical Sciences, Monash University, Clayton, Victoria, Australia ; The ARC Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton, Victoria, Australia.
PLoS One. 2013 Oct 21;8(10):e78357. doi: 10.1371/journal.pone.0078357. eCollection 2013.
It has recently been recognised that vaccine adjuvants play a critical role in directing the nature of a vaccine induced effector response. In the present study, several adjuvants were evaluated for their ability to protect sheep after field vaccination with the larval-specific Haemonchus contortus antigen, HcsL3. Using a suboptimal antigen dose, aluminium adjuvant was shown to reduce the cumulative faecal egg counts (cFEC) and worm burden by 23% and 25% respectively, in agreement with a previous study. The addition of Quil A to the aluminium-adjuvanted vaccine brought cFEC back to control levels. Vaccination with the adjuvant DEAE-dextran almost doubled the protection compared to the aluminium-adjuvanted vaccine resulting in 40% and 41% reduction in cFEC and worm counts compared to controls. Examination of skin responses following i.d. injection of exsheathed L3, revealed that cFEC was negatively correlated with wheal size and tissue eosinophils for the DEAE-dextran and aluminium-adjuvanted groups respectively. These studies have for the first time shown the potential of DEAE-dextran adjuvant for helminth vaccines, and discovered significant cellular correlates of vaccine-induced protection.
最近人们认识到，疫苗佐剂在引导疫苗诱导的效应反应性质方面起着关键作用。在本研究中，评估了几种佐剂在用幼虫特异性捻转血矛线虫抗原HcsL3进行现场疫苗接种后保护绵羊的能力。使用次优抗原剂量时，铝佐剂显示分别使累积粪虫卵计数（cFEC）和虫负荷降低了23%和25%，这与之前的一项研究结果一致。在铝佐剂疫苗中添加Quil A使cFEC恢复到对照水平。与铝佐剂疫苗相比，用佐剂二乙氨基乙基葡聚糖（DEAE-葡聚糖）进行疫苗接种的保护效果几乎翻倍，与对照相比，cFEC和虫数分别减少了40%和41%。皮下注射脱鞘L3后对皮肤反应的检查显示，对于DEAE-葡聚糖组和铝佐剂组，cFEC分别与风团大小和组织嗜酸性粒细胞呈负相关。这些研究首次表明了DEAE-葡聚糖佐剂用于蠕虫疫苗的潜力，并发现了疫苗诱导保护的重要细胞相关因素。
