通过酶联免疫吸附测定法（ELISA）评估用于诊断绵羊血矛线虫病的不同抗原及其与其他蠕虫的交叉反应性。
Evaluation of different antigens for diagnosis of sheep haemonchosis by ELISA and their cross reactivity with other helminthes.
作者信息
Kandil Omnia M, Hendawy Seham H M, El Namaky Amira H, Gabrashanska Margarita P, Nanev Veselin N
机构信息
Department of Parasitology and Animal Diseases, National Research Centre, El- Bohouth Street, Dokki, Post Box 12622, Giza, Egypt.
Department of Experimental Parasitology, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev St. Bl.25, 1113 Sofia, Bulgaria.
出版信息
J Parasit Dis. 2017 Sep;41(3):678-683. doi: 10.1007/s12639-016-0865-3. Epub 2016 Nov 23.
() remains important nematode that infecting sheep all over the world. Truthful diagnosis of haemonchosis needs reliable Enzyme linked immune sorbent assay test as well as the immuno-reactive protein profile of different prepared antigens; larval (L), excretory secretory product (ESP) and adult somatic (AS). The current study fulfilled that L antigen is the talented antigen for such serological diagnosis. Immunodominant band at molecular weight 57 kDa were answerable for highest specificity and accuracy of positive predictive value of this antigen. Moreover, the highest apparent prevalence value was 92 and 75% obtained by L and ESP antigens, respectively. The results of cross reactivity among AS () and spp. revealed that AS antigen appeared major cross reactivity with other cestode and trematode. Best dilution of serum was (1:800) to rise above this phenomenon.
（）仍然是一种重要的线虫，感染着世界各地的绵羊。准确诊断捻转血矛线虫病需要可靠的酶联免疫吸附测定试验以及不同制备抗原（幼虫（L）、排泄分泌产物（ESP）和成虫体细胞（AS））的免疫反应蛋白谱。当前研究证实L抗原是用于此类血清学诊断的优质抗原。分子量为57 kDa的免疫显性带负责该抗原最高的特异性和阳性预测值的准确性。此外，L和ESP抗原分别获得的最高表观流行率值为92%和75%。AS（）和其他物种之间的交叉反应结果表明，AS抗原与其他绦虫和吸虫表现出主要交叉反应。血清的最佳稀释度为（1:800）以克服这一现象。
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