Razzaq Abdul, Ashraf Kamran, Maqbool Azhar, Islam Muhammad, Hanan Abdul, Awais Mian Muhammad, Khetran Munir Ahmad, Jan Saadullah, Shafee Muhammad, Essa Muhammad, Kakar Hamdullah
Animal Sciences Research Program, Balochistan Agricultural Research and Development Centre, PARC, Brewery Road, Quetta, Pakistan.
Dept. of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Iran J Parasitol. 2014 Apr-Jun;9(2):169-80.
Among the infectious organisms of parasitic origin, gastrointestinal nematodes are very important as they have been reported worldwide. The main aim of the present research study to highlight the annual epidemiological contributing factors associated with the prevalence of gastrointestinal nematodes and their control in sheep.
A total 1200 faecal samples (100 per month) were collected from farmers holding Balochi-sheep (either sexes, 1-5 years old) during January-December 2012 and analyzed to determine the prevalence of nematodes based on microscopy and ELISA based diagnostic assay. Therapeutic efficacies of different synthetic and herbal medicines against these nematodes were assessed by field trials.
Results showed that 23.92% Balochi-sheep were infected with nematodes. Five nematodes infections were recorded with highest prevalence of Haemonchus (7.75%) followed by Nematodirus (7.58%), Strongyloides (4.42%), Trichostrongylus (2.33%) and Trichuris (1.83%). The younger and older ewes (one and five years) presented higher nematodes prevalence with peak during March/April and August/September. Haemonchus and Trichuris positive samples based on coprological examination were also showed 92-100% positive sensitivity for these nematodes by the ELISA. Sheep treated with Ivermectin showed higher reduction (97.76%) in nematode egg counts followed by Atreefal deedan (96.42%) and Oxfendazole (95.44%), respectively.
The gastro-intestinal nematodes are prevalent in all age and either sex of Balochi-sheep with peak during summer. The ELISA based diagnosis is more accurte. The synthetic and herbal products are very effective against sheep nematodes.
在寄生虫源性感染生物中，胃肠道线虫非常重要，因为它们在全球范围内均有报道。本研究的主要目的是突出与绵羊胃肠道线虫流行及其控制相关的年度流行病学影响因素。
2012年1月至12月期间，从饲养俾路支羊（1至5岁，不分性别）的农户中总共采集了1200份粪便样本（每月100份），并基于显微镜检查和酶联免疫吸附测定（ELISA）诊断方法进行分析，以确定线虫的流行情况。通过田间试验评估了不同合成药物和草药对这些线虫的治疗效果。
结果显示，23.92%的俾路支羊感染了线虫。记录到五种线虫感染，其中血矛线虫的流行率最高（7.75%），其次是细颈线虫（7.58%）、类圆线虫（4.42%）、毛圆线虫（2.33%）和鞭虫（1.83%）。年轻和年老的母羊（1岁和5岁）线虫流行率较高，在3月/4月和8月/9月达到峰值。基于粪便学检查的血矛线虫和鞭虫阳性样本通过ELISA检测对这些线虫也显示出92 - 100%的阳性敏感性。用伊维菌素治疗的绵羊线虫卵计数减少幅度更高（97.76%），其次是阿特里法尔迪丹（96.42%）和奥芬达唑（95.44%）。
胃肠道线虫在俾路支羊的所有年龄和性别中均有流行，在夏季达到峰值。基于ELISA的诊断更准确。合成产品和草药产品对绵羊线虫非常有效。
