U.S. Department of Agriculture, Agricultural Research Service, Food Safety and Intervention Technologies Research Unit, James W. W. Baker Center, Delaware State University, Dover, DE, 19901, USA.
Department of Animal and Food Sciences, University of Delaware, Newark, DE, 19716-2150, USA.
Food Environ Virol. 2018 Mar;10(1):83-88. doi: 10.1007/s12560-017-9315-3. Epub 2017 Aug 22.
Male-specific bacteriophages have been proposed as human enteric virus indicators for shellfish. In this study, Eastern oysters (Crassostrea virginica) were individually exposed to 5.6 × 10 PFU of MS-2 for 48 h at 15 °C followed by collective maintenance in continuously UV-sterilized seawater for 0-6 weeks at either 7, 15, or 24 °C. Initial contamination levels of MS-2 were >6 log PFU. Assessment of weekly declines of viable MS-2 indicated that cooler temperatures dramatically enhanced the persistence of MS-2 within oyster tissues. At 3 weeks, the average log PFU reductions for MS-2 within oysters were 2.28, 2.90, and 4.57 for oysters held at 7, 15, and 24 °C, respectively. Fitting temporal survival data with linear and nonlinear Weibull models indicated that the Weibull model best fit the observed reductions. In total, these data can serve as a guideline for regulatory agencies regarding the influence of water temperature on indicator phage after episodic sewage exposure.
已有人提议将雄性特异性噬菌体作为贝类的人类肠道病毒指示物。在这项研究中，东方牡蛎（Crassostrea virginica）在 15°C 下单独暴露于 5.6×10 PFU 的 MS-2 中 48 小时，然后在 7、15 或 24°C 下在连续紫外线消毒的海水中集体维持 0-6 周。MS-2 的初始污染水平>6 log PFU。每周评估 MS-2 的存活情况表明，较冷的温度极大地增强了 MS-2 在牡蛎组织中的持久性。3 周时，7、15 和 24°C 下的牡蛎中 MS-2 的平均对数 PFU 减少分别为 2.28、2.90 和 4.57。用线性和非线性 Weibull 模型拟合时间存活数据表明，Weibull 模型最适合观察到的减少。总的来说，这些数据可以作为监管机构的指南，了解在偶发性污水暴露后水温对指示噬菌体的影响。
