Allwood Paul B, Malik Yashpal S, Hedberg Craig W, Goyal Sagar M
Division of Environmental and Occupational Health, School of Public Health, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Appl Environ Microbiol. 2003 Sep;69(9):5707-10. doi: 10.1128/AEM.69.9.5707-5710.2003.
The relationship between the survival of enteric viral pathogens and their indicators (coliform bacteria and coliphages) is not well understood. We compared the survival rates of feline calicivirus (FCV), Escherichia coli, and a male-specific RNA coliphage MS2 at 4, 25, and 37 degrees C for up to 28 days in dechlorinated water. The survival rates of E. coli and FCV, a surrogate of noroviruses (NV), had a high degree of correlation at 4 and 25 degrees C, while MS2 phage survived significantly longer (P < 0.05) at these two temperatures. At 37 degrees C, the survival rates for all three organisms were highly correlated. Decimal reduction values indicating the number of days needed for 90% reduction in titer (D values) decreased for all three organisms as storage temperatures increased. FCV had the shortest D value among all three organisms at all temperatures investigated. These findings indicate that F-specific RNA phages may be useful indicators of NV in the environment.
肠道病毒病原体的存活与其指标（大肠菌群和大肠杆菌噬菌体）之间的关系尚未完全明确。我们比较了猫杯状病毒（FCV）、大肠杆菌和雄性特异性RNA噬菌体MS2在4℃、25℃和37℃的脱氯水中长达28天的存活率。在4℃和25℃时，大肠杆菌和作为诺如病毒（NV）替代物的FCV的存活率具有高度相关性，而MS2噬菌体在这两个温度下存活时间显著更长（P<0.05）。在37℃时，所有三种生物体的存活率高度相关。随着储存温度升高，所有三种生物体的表示滴度降低90%所需天数的十进制减少值（D值）均下降。在所研究的所有温度下，FCV在所有三种生物体中D值最短。这些发现表明，F特异性RNA噬菌体可能是环境中NV的有用指标。
