Korhonen T K, Kalkkinen N, Haahtela K, Old D C
J Bacteriol. 1987 May;169(5):2281-3. doi: 10.1128/jb.169.5.2281-2283.1987.
Type 1 fimbriae from Erwinia carotovora subsp. carotovora and mannose-resistant fimbriae from Erwinia rhapontici were purified and characterized. The type 1 fimbrillin had an apparent molecular weight of 16,500; that of the mannose-resistant fimbrillin was 18,000. The amino-terminal amino acid sequences of the two fimbrillins were related, but tryptic peptide maps showed significant differences between the proteins. No serological cross-reaction was found between the two fimbrial filaments, nor did they cross-react with type 1 or type 3 fimbriae purified from other enterobacterial species. Immunofluorescent staining of bacterial populations revealed that they were heterogeneous with respect to fimbriation.
胡萝卜软腐欧文氏菌胡萝卜软腐亚种的1型菌毛和甜菜欧文氏菌的甘露糖抗性菌毛被纯化并进行了特性分析。1型菌毛蛋白的表观分子量为16,500；甘露糖抗性菌毛蛋白的表观分子量为18,000。两种菌毛蛋白的氨基末端氨基酸序列相关，但胰蛋白酶肽图谱显示这两种蛋白质之间存在显著差异。两种菌毛丝之间未发现血清学交叉反应，它们也不与从其他肠杆菌科物种纯化的1型或3型菌毛发生交叉反应。对细菌群体的免疫荧光染色显示，它们在菌毛形成方面存在异质性。
