Korhonen T K, Väisänen V, Saxén H, Hultberg H, Svenson S B
Infect Immun. 1982 Jul;37(1):286-91. doi: 10.1128/iai.37.1.286-291.1982.
P-antigen-recognizing fimbriae (P fimbriae) from four pyelonephritogenic Escherichia coli strains and type 1 fimbriae from an E. coli strain and a Salmonella typhimurium strain were purified. The P fimbriae were morphologically similar to type 1 fimbriae. The purified P fimbriae agglutinated neuraminidase-treated human P1 and P2k erythrocytes but not p erythrocytes, which lack all P-blood group-specific glycosphingolipids. However, coating of neuraminidase-treated p erythrocytes with globoside rendered such erythrocytes agglutinable by the P fimbriae. The hemagglutinations were in all instances fully inhibited by the synthetic alpha-D-Galp-(1-4)-beta-D-Galp-1-O-Me glycoside. The binding specificity of the P fimbriae could also be demonstrated by using fimbriae coated onto latex particles and nontreated erythrocytes. It was thus concluded that the P fimbriae recognize and bind to the alpha-D-Galp-(1-4)-beta-D-Galp carbohydrate sequence occurring in the series of P-blood group antigen-specific glycosphingolipids. In contrast to both type 1 fimbriae, all four P fimbriae preparations showed multiple bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Antisera were raised in rabbits against the various E. coli fimbriae. In enzyme-linked immunosorbent assays each one of the antisera to the P fimbriae reacted to titers of log 4 to 7 with both the homologous and the heterologous P fimbriae, but not with the type 1 fimbriae of E. coli. In a reciprocal fashion, the antiserum to the type 1 fimbriae of one E. coli strain reacted only with the homologous type 1 but not with any of the P fimbriae preparations.
从四株引起肾盂肾炎的大肠杆菌菌株中纯化出了识别P抗原的菌毛（P菌毛），并从一株大肠杆菌菌株和一株鼠伤寒沙门氏菌菌株中纯化出了1型菌毛。P菌毛在形态上与1型菌毛相似。纯化后的P菌毛能凝集经神经氨酸酶处理的人P1和P2k红细胞，但不能凝集缺乏所有P血型特异性糖鞘脂的p红细胞。然而，用红细胞糖苷脂包被经神经氨酸酶处理的p红细胞后，这种红细胞能被P菌毛凝集。所有情况下，血凝反应均被合成的α-D-半乳糖-(1-4)-β-D-半乳糖-1-O-甲基糖苷完全抑制。通过使用包被在乳胶颗粒上的菌毛和未处理的红细胞，也能证明P菌毛的结合特异性。因此得出结论，P菌毛识别并结合存在于一系列P血型抗原特异性糖鞘脂中的α-D-半乳糖-(1-4)-β-D-半乳糖碳水化合物序列。与1型菌毛不同的是，所有四种P菌毛制剂在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中均显示出多条带。用兔制备了针对各种大肠杆菌菌毛的抗血清。在酶联免疫吸附试验中，每种针对P菌毛的抗血清与同源和异源P菌毛的反应滴度为log 4至7，但不与大肠杆菌的1型菌毛反应。反之，一株大肠杆菌菌株的1型菌毛抗血清仅与同源的1型菌毛反应，而不与任何一种P菌毛制剂反应。
