Metha K, Turpin J, Lopez-Berestein G
J Leukoc Biol. 1987 Apr;41(4):341-8. doi: 10.1002/jlb.41.4.341.
Human peripheral blood monocytes (HPBM) from normal donors, isolated by counter-current centrifugal elutriation into two subpopulations, showed no significant difference in their ability to differentiate in vitro into macrophages as determined by induction of a protein cross-linking enzyme tissue transglutaminase (TGase). The two subpopulations were equally responsive to the augmenting effect of recombinant interferon-gamma (rIFN-gamma) on expression of tissue TGase. In vitro maturation and treatment with rIFN-gamma of HPBM were associated with increased binding of tritiated retinol. Intracellular delivery of retinol rendered this hormone active in inducing the differentiation of HPBM. The retinoid-induced expression of tissue TGase was the result of increased accumulation of the enzyme peptide and not activation of preexisting enzyme. We propose, therefore, that maturation of HPBM, induced by in vitro culture or treatment with rIFN-gamma, is associated with acquisition of cell surface receptors for serum retinol-binding protein.
通过逆流离心淘析法从正常供体中分离出的人外周血单核细胞（HPBM）被分为两个亚群，通过诱导蛋白交联酶组织转谷氨酰胺酶（TGase）测定，这两个亚群在体外分化为巨噬细胞的能力上没有显著差异。这两个亚群对重组干扰素-γ（rIFN-γ）对组织TGase表达的增强作用具有同等反应性。HPBM的体外成熟和用rIFN-γ处理与氚标记视黄醇的结合增加有关。视黄醇的细胞内递送使这种激素在诱导HPBM分化中具有活性。类视黄醇诱导的组织TGase表达是酶肽积累增加的结果，而不是预先存在的酶的激活。因此，我们提出，体外培养或用rIFN-γ处理诱导的HPBM成熟与获得血清视黄醇结合蛋白的细胞表面受体有关。
