Piccirillo Nicola, Putzulu Rossana, Massini Giuseppina, Fiore Assunta Gessica, Chiusolo Patrizia, Sica Simona, Zini Gina
Transfusion Medicine Department, Catholic University of Rome, Rome, Italy.
Haematology Department, Catholic University of Rome, Rome, Italy.
J Clin Apher. 2018 Jun;33(3):217-221. doi: 10.1002/jca.21574. Epub 2017 Aug 22.
Extracorporeal photopheresis (ECP) is the most represented cell therapy for treatment of cutaneous T-cell lymphoma, graft-versus host disease and organ rejection. We analyzed our experience in ECP using 2 cell separators (Cobe Spectra and Spectra Optia) focusing on leukapheretic product characteristics, UV-A irradiation procedure and entire ECP process.
We collected data of patients undergoing ECP between January 2012 and February 2015 in order to evaluate collection procedures performed using Cobe Spectra and Spectra Optia, mononuclear cell product, UV-A photoirradiation procedure by Pit System.
We performed 484 ECP procedures in 27 patients. Cobe-derived mononuclear cell products were characterized by higher cell yields while Optia-derived mononuclear cell products were characterized by smaller volume, comparable mononuclear cell content but lower erythrocytes, granulocytes, and platelets contamination.
Our study confirms good results for both cell separators. Blood volume processed being equal, Cobe collects a number of total nucleated cells significantly higher than Optia. Optia, collecting only target cells without significant erythrocytes, granulocytes and platelets contamination, is able to collect a leukapheretic product particularly suitable for ECP.
体外光化学疗法（ECP）是治疗皮肤T细胞淋巴瘤、移植物抗宿主病和器官排斥反应最具代表性的细胞疗法。我们分析了使用两种细胞分离仪（Cobe Spectra和Spectra Optia）进行ECP的经验，重点关注白细胞分离产物特征、紫外线A照射程序和整个ECP过程。
我们收集了2012年1月至2015年2月期间接受ECP治疗的患者的数据，以评估使用Cobe Spectra和Spectra Optia进行的采集程序、单个核细胞产物以及通过Pit系统进行的紫外线A光照射程序。
我们对27例患者进行了484次ECP治疗。Cobe分离的单个核细胞产物的特点是细胞产量较高，而Optia分离的单个核细胞产物的特点是体积较小，单个核细胞含量相当，但红细胞、粒细胞和血小板污染较低。
我们的研究证实了两种细胞分离仪都有良好的效果。在处理血量相同的情况下，Cobe采集的总核细胞数量明显高于Optia。Optia仅收集目标细胞，且无明显的红细胞、粒细胞和血小板污染，能够收集到特别适合ECP的白细胞分离产物。
