Kartal Orkan, Laner-Plamberger Sandra, Rohde Eva, Mrazek Cornelia, Lauth Wanda, Grabmer Christoph
Department for Transfusion Medicine, University Hospital of Salzburg, Paracelsus Medical University Salzburg, Muellner Hauptstraße 48, 5020 Salzburg, Austria.
GMP Laboratory, Paracelsus Medical University Salzburg, Strubergasse 21, 5020 Salzburg, Austria.
Diagnostics (Basel). 2024 Oct 15;14(20):2290. doi: 10.3390/diagnostics14202290.
: Extracorporeal photopheresis (ECP) is a well-established and efficacious cell therapy for a range of diseases. The objective of this retrospective study was to compare the new Amicus Blue inline system with the Therakos Cellex inline system and the Spectra Optia offline system in terms of collection efficiency, mononuclear cell (MNC) yield of mononuclear cell products (MCPs), processing time and correlation between MCP cell count and peripheral blood count of patients.
This retrospective study compared 127 procedures utilizing the Spectra Optia offline system, 93 procedures employing the Amicus Blue inline system, and 81 procedures applying the Therakos Cellex inline system. The MNCs were subjected to flow cytometry analysis for CD45 and CD14 expression in order to ascertain the precise composition of the collected lymphocyte and monocyte fractions.
The Therakos inline system demonstrated the highest MNC collection efficiency (Therakos: 74.42 ± 1.82; Optia: 65.79 ± 1.48; Amicus: 56.32 ± 2.80; < 0.01). Regarding the content of collected MNCs (×10⁶/kg body weight), the Spectra Optia offline system was superior to the other systems (Optia: 42.69 ± 1.42; Therakos: 31.21 ± 1.66; Amicus: 27.56 ± 1.54; < 0.01).
This study represents the first direct comparison of the new Amicus Blue inline system with the two most commonly used ECP systems in the same patient cohort of a single center. The data show that the Amicus Blue inline system collects sufficient MNCs to perform an ECP, but it has a significantly lower CE than the other systems and a significantly lower amount of collected MNCs than the Spectra Optia offline system.
体外光化学疗法（ECP）是一种针对多种疾病的成熟且有效的细胞疗法。本回顾性研究的目的是在采集效率、单核细胞产品（MCP）的单核细胞（MNC）产量、处理时间以及MCP细胞计数与患者外周血计数之间的相关性方面，将新型Amicus Blue在线系统与Therakos Cellex在线系统和Spectra Optia离线系统进行比较。
本回顾性研究比较了127例使用Spectra Optia离线系统的操作、93例采用Amicus Blue在线系统的操作以及81例应用Therakos Cellex在线系统的操作。对MNC进行流式细胞术分析，以确定所采集淋巴细胞和单核细胞组分的精确组成。
Therakos在线系统显示出最高的MNC采集效率（Therakos：74.42±1.82；Optia：65.79±1.48；Amicus：56.32±2.80；<0.01）。关于所采集MNC的含量（×10⁶/kg体重），Spectra Optia离线系统优于其他系统（Optia：42.69±1.42；Therakos：31.21±1.66；Amicus：27.56±1.54；<0.01）。
本研究是在单一中心的同一患者队列中，首次对新型Amicus Blue在线系统与两种最常用的ECP系统进行直接比较。数据表明，Amicus Blue在线系统能够采集到足够的MNC来进行ECP，但与其他系统相比，其采集效率显著较低，且所采集的MNC数量比Spectra Optia离线系统显著更少。
