Core Facility Hard Tissue and Biomaterial Research, Karl Donath Laboratory, School of Dentistry, Medical University of Vienna, Vienna, Austria.
Austrian Cluster for Tissue Regeneration, Vienna, Austria.
J Clin Periodontol. 2017 Dec;44(12):1226-1235. doi: 10.1111/jcpe.12799. Epub 2017 Nov 17.
Tumour necrosis factor (TNF)-α is a pathological factor causing the characteristic symptoms of periodontal disease and rheumatoid arthritis. In this study, we describe the phenotypes of human TNF-α transgenic mice (hTNFtg) with respect to their periodontium and the temporomandibular joint (TMJ).
Periodontal structures, TMJ and skull shape of hTNFtg mice and wild-type (WT) littermates were assessed by microcomputed tomography, automated segmentation, geometric morphometrics and histologic ground sections.
We show that hTNFtg mice have an eroded lamina dura and reduced periodontal ligament space compared to (WT) littermates. Transgenic mice further exhibit severe destruction of the TMJ. Geometric morphometrics revealed that hTNFtg mice have a more laterally positioned TMJ with a concomitantly enlarged zygomatic process. Mandibular and maxillary teeth occluded properly.
Our findings suggest that chronic inflammation in hTNFtg mice causes destructive changes of the periodontium and the TMJ.
肿瘤坏死因子（TNF）-α是一种病理性因子，可引起牙周病和类风湿性关节炎的特征性症状。本研究描述了人 TNF-α转基因小鼠（hTNFtg）在牙周组织和颞下颌关节（TMJ）方面的表型。
通过 microCT、自动分割、几何形态计量学和组织学地面切片评估 hTNFtg 小鼠和野生型（WT）同窝仔鼠的牙周结构、TMJ 和颅骨形状。
我们发现 hTNFtg 小鼠的硬骨板被侵蚀，牙周韧带空间缩小，与（WT）同窝仔鼠相比。转基因小鼠的 TMJ 进一步受到严重破坏。几何形态计量学显示，hTNFtg 小鼠的 TMJ 位置更偏向外侧，同时颧骨突增大。下颌骨和上颌骨牙齿正常咬合。
我们的研究结果表明，hTNFtg 小鼠的慢性炎症导致牙周组织和 TMJ 的破坏性变化。
