Shiraishi Katsunori, Kamei Naosuke, Takeuchi Shunsuke, Yanada Shinobu, Mera Hisashi, Wakitani Shigeyuki, Adachi Nobuo, Ochi Mitsuo
Department of Orthopaedic Surgery, Division of Medicine, Biomedical Sciences Major, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
Medical Center for Translational & Clinical Research, Hiroshima University Hospital, Hiroshima, Japan.
Stem Cells Int. 2017;2017:8740294. doi: 10.1155/2017/8740294. Epub 2017 Aug 1.
Quality evaluation of mesenchymal stem cells (MSCs) based on efficacy would be helpful for their clinical application. In this study, we aimed to find the factors of human bone marrow MSCs relating to cartilage repair. The expression profiles of humoral factors, messenger RNAs (mRNAs), and microRNAs (miRNAs) were analyzed in human bone marrow MSCs from five different donors. We investigated the correlations of these expression profiles with the capacity of the MSCs for proliferation, chondrogenic differentiation, and cartilage repair in vivo. The mRNA expression of was positively correlated with proliferation and cartilage differentiation. By contrast, the mRNA expression of and the protein expression of TIMP-1 and VEGF were negatively correlated with proliferation and cartilage differentiation. However, MSCs from all five donors had the capacity to promote cartilage repair in vivo regardless of their capacity for proliferation and cartilage differentiation. The mRNA expression of was positively correlated with cartilage repair in vivo. Meanwhile, the mRNA expression of and expression of miR-486-3p, miR-148b, miR-93, and miR-320B were negatively correlated with cartilage repair. The expression analysis of these factors might help to predict the ability of bone marrow MSCs to promote cartilage repair.
基于疗效对间充质干细胞（MSCs）进行质量评估将有助于其临床应用。在本研究中，我们旨在找出与人类骨髓间充质干细胞软骨修复相关的因素。分析了来自五个不同供体的人类骨髓间充质干细胞中体液因子、信使核糖核酸（mRNAs）和微小核糖核酸（miRNAs）的表达谱。我们研究了这些表达谱与间充质干细胞在体内的增殖、软骨分化及软骨修复能力之间的相关性。[此处原文缺失具体基因名称]的mRNA表达与增殖和软骨分化呈正相关。相比之下，[此处原文缺失具体基因名称]的mRNA表达以及TIMP - 1和VEGF的蛋白表达与增殖和软骨分化呈负相关。然而，来自所有五个供体的间充质干细胞无论其增殖和软骨分化能力如何，均具有在体内促进软骨修复的能力。[此处原文缺失具体基因名称]的mRNA表达与体内软骨修复呈正相关。同时，[此处原文缺失具体基因名称]的mRNA表达以及miR - 486 - 3p、miR - 148b、miR - 93和miR - 320B的表达与软骨修复呈负相关。这些因素的表达分析可能有助于预测骨髓间充质干细胞促进软骨修复的能力。
