Clinical Cytology and STD Laboratory, Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, PR, Brazil.
Sperm Analysis Section, São Camilo Laboratory, Maringá, PR, Brazil.
Biomed Res Int. 2017;2017:4684629. doi: 10.1155/2017/4684629. Epub 2017 Aug 1.
Several studies have addressed the impact of viral infections on male infertility. However, it is still unknown whether human papillomavirus (HPV) can alter seminal parameters. The aim of this study was to determine the prevalence of HPV in the semen of male partners of couples seeking fertility evaluation. Additionally, we assessed the possibility that HPV infections affect seminal parameters. A total of 229 semen samples were collected from men in the Sperm Analysis Section of São Camilo Laboratory of Maringá, Brazil, between October 2015 and March 2016. Basic seminal parameters were analyzed, and HPV was detected and genotyped by polymerase chain reaction. HPV DNA was detected in 16.6% of samples. Of these, 10.5% had single type HPV infections, 6.1% had multiple HPV infections, 5.7% had exclusively high-risk HPV, and 6.1% had exclusively low-risk HPV. Samples positive for single and multiple types of HPV were associated with abnormal viscosity, and samples positive for multiple HPV types were also associated with hypospermia, higher pH, and increased leukocyte numbers. These findings suggest that the male partners of infertile couples with seminal HPV infections may have prostate disturbances indicative of glandular dysfunction, which may influence fertility.
一些研究已经探讨了病毒感染对男性不育的影响。然而，目前仍不清楚人乳头瘤病毒（HPV）是否会改变精液参数。本研究旨在确定在寻求生育评估的夫妇中男性伴侣的精液中 HPV 的流行率。此外，我们评估了 HPV 感染是否会影响精液参数的可能性。本研究共收集了 229 份来自巴西马兰加 São Camilo 实验室精子分析科男性的精液样本，收集时间为 2015 年 10 月至 2016 年 3 月。分析了基本精液参数，并通过聚合酶链反应检测和 HPV 基因分型。在 16.6%的样本中检测到 HPV DNA。其中，10.5%的样本为单一类型 HPV 感染，6.1%的样本为多种 HPV 感染，5.7%的样本为单纯高危型 HPV 感染，6.1%的样本为单纯低危型 HPV 感染。单一和多种类型 HPV 阳性的样本与异常黏度有关，而多种 HPV 类型阳性的样本也与少精症、较高 pH 值和白细胞数量增加有关。这些发现表明，患有精液 HPV 感染的不育夫妇的男性伴侣可能存在前列腺功能障碍，这可能会影响生育能力。
