Kurscheidt Fábio A, Damke Edilson, Bento Jaqueline C, Balani Valério A, Takeda Karen I, Piva Sérgio, Piva João P, Irie Mary M T, Gimenes Fabrícia, Consolaro Marcia E L
Programa de Pós-Graduação em Biociências e Fisiopatologia, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil; Sperm Analysis Section, São Camilo Laboratory, Maringá, Paraná, Brazil.
Programa de Pós-Graduação em Biociências e Fisiopatologia, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil.
Urology. 2018 Mar;113:52-58. doi: 10.1016/j.urology.2017.11.050. Epub 2017 Dec 26.
To assess the possibility that herpes simplex virus (HSV) infection could affect seminal parameters, we (1) standardize and validate a multiplex polymerase chain reaction (M-PCR) assay to detect HSV-1 and HSV-2 in semen, and (2) determine the prevalence of HSV-1/-2 in the semen of randomized male partners of couples seeking fertility evaluation.
A total of 279 semen samples were included in the study from men who visited the Sperm Analysis Section of São Camilo Laboratory of Maringá, Brazil, between November 2014 and July 2015. Eligible men were 18 years or older and had a semen analysis due fertility evaluation (after failing to conceive with their partner after 1 year of unprotected intercourse). Basic seminal parameters were analyzed, and HSV-1 and HSV-2 were detected by M-PCR.
The M-PCR assay clearly distinguished and identified 2 HSV types in semen samples. HSV in total was detected in 10.7% of samples, of which 7.5% had HSV-1 exclusively and 3.2% had HSV-2 exclusively. We detected a significant association of HSV-2 infection with hematospermia and with a lower mean seminal volume, and between HSV-1 infection and a lower mean sperm count.
These findings suggest that the male partners of infertile couples with HSV infections may have changes on the 2 equally important components of semen, spermatozoa and seminal fluid, which may influence fertility. Further studies enrolling a larger number of patients are necessary to confirm these data and to elucidate the clinical relevance of HSV presence in semen.
为评估单纯疱疹病毒（HSV）感染是否会影响精液参数，我们（1）标准化并验证一种多重聚合酶链反应（M-PCR）检测方法，以检测精液中的HSV-1和HSV-2，以及（2）确定寻求生育评估的夫妇中随机选取的男性伴侣精液中HSV-1/-2的流行率。
2014年11月至2015年7月期间，共有279份精液样本纳入了该研究，这些样本来自巴西马林加圣卡米洛实验室精子分析科的男性。符合条件的男性年龄在18岁及以上，因生育评估接受精液分析（在未采取保护措施性交1年后未能与伴侣受孕）。分析基本精液参数，并通过M-PCR检测HSV-1和HSV-2。
M-PCR检测方法能够清晰地区分和鉴定精液样本中的2种HSV类型。总共在10.7%的样本中检测到HSV，其中7.5%仅感染HSV-1，3.2%仅感染HSV-2。我们检测到HSV-2感染与血精症以及较低的平均精液量之间存在显著关联，并且HSV-1感染与较低的平均精子计数之间存在显著关联。
这些发现表明，患有HSV感染的不育夫妇的男性伴侣精液中同样重要的两个组成部分，即精子和精液，可能会发生变化，这可能会影响生育能力。需要进一步纳入更多患者的研究来证实这些数据，并阐明精液中HSV存在的临床相关性。
