Tewari S, Brown E C, Gaultier C R, Najarian P
Neurochem Res. 1987 Feb;12(2):179-88. doi: 10.1007/BF00979535.
Present studies on the sensitivity of the transcription process in rat cerebral hemispheres showed that the relative abundance and translation of free and bound poly (A)+ mRNAs in a reticulocyte mRNA-dependent system were reduced following 1 h and 4 h of ethanol or pentobarbital administration with free being affected to a greater extent than the bound poly (A)+ mRNAs. In addition, the energy-dependent nucleocytoplasmic transport of in vivo [3H]labeled nuclear RNA to surrogate cytoplasm was modified in response to ethanol exposure. The translocation of the labeled nuclear RNA fraction occurred only to the microsomal/ribosomal fractions of the cytoplasm, was stimulated by cyclic cAMP and abolished when the cytoplasm was depleted of its protein factors following streptomycin treatment, thereby establishing the translocated RNA as messenger RNA. It is concluded that the neural cell, in response to ethanol exposure, modifies the efficiency of nuclear processing and transport of mRNA. This nuclear restriction probably occurs at multi-levels during the post-transcriptional modification of mRNAs.
目前关于大鼠大脑半球转录过程敏感性的研究表明，在网织红细胞mRNA依赖性系统中，乙醇或戊巴比妥给药1小时和4小时后，游离和结合的多聚（A）+ mRNA的相对丰度和翻译均降低，其中游离的多聚（A）+ mRNA受影响的程度大于结合的多聚（A）+ mRNA。此外，体内[3H]标记的核RNA向替代细胞质的能量依赖性核质转运因乙醇暴露而发生改变。标记的核RNA部分仅转运至细胞质的微粒体/核糖体部分，受环磷酸腺苷刺激，当用链霉素处理使细胞质中的蛋白质因子耗尽时则转运停止，从而确定转运的RNA为信使RNA。得出的结论是，神经细胞在接触乙醇后，会改变mRNA的核加工和转运效率。这种核限制可能发生在mRNA转录后修饰的多个层面。
