Department of Chemistry, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
Biol Trace Elem Res. 2018 May;183(1):58-70. doi: 10.1007/s12011-017-1137-5. Epub 2017 Aug 23.
The present study is based on the measurement of concentrations of selected trace metals (Fe, Zn, Cu, Co, Mn, Ni, Cr, Cd and Pb) and thyroid hormones (TSH and T) in blood serum of hypothyroid and hyperthyroid patients in comparison with healthy donors/controls in order to establish the imbalances of the trace metals in diseased subjects. The serum samples were digested in HNO-HClO mixture and quantification of the metals was performed by flame atomic absorption spectrometry. Average levels of Fe, Ni, Cu, Cr, Pb and TSH were found to be significantly higher (p < 0.05) in the serum of hypothyroid patients compared with other donor categories, while mean concentrations of Mn, Cd and T were significantly elevated in the serum of hyperthyroid patients compared with other donor groups (p < 0.05). The correlation pattern of trace metals in the serum of patient groups revealed significantly different mutual associations compared with the controls. PCA and CA pointed out the interferences of the toxic metals with essential metals in the serum of both patient groups compared with the controls. Most of the metals exhibited noticeable disparities in their concentrations based on gender, food habits and tobacco use for all donor groups. Thus, the pathogenesis of thyroid diseases is significantly affecting the essential trace and toxic metals balance in both patients groups.
本研究基于对甲状腺功能减退症和甲状腺功能亢进症患者血清中选定微量元素（铁、锌、铜、钴、锰、镍、铬、镉和铅）和甲状腺激素（TSH 和 T）浓度的测量，与健康供体/对照进行比较，以确定患病个体中微量元素的失衡。血清样品用 HNO-HClO 混合物消化，通过火焰原子吸收光谱法对金属进行定量。与其他供体类别相比，甲状腺功能减退症患者血清中的铁、镍、铜、铬、铅和 TSH 平均水平显著升高（p < 0.05），而甲状腺功能亢进症患者血清中的锰、镉和 T 平均浓度与其他供体组相比明显升高（p < 0.05）。与对照组相比，患者组血清中微量元素的相关模式显示出明显不同的相互关系。PCA 和 CA 指出，与对照组相比，两组患者血清中的有毒金属与必需金属之间存在干扰。对于所有供体组，基于性别、饮食习惯和吸烟，大多数金属的浓度都存在明显差异。因此，甲状腺疾病的发病机制显著影响两组患者的必需痕量和有毒金属平衡。
