Zhang Long, Dong Yongbin, Wang Qilei, Du Chunguang, Xiong Wenwei, Li Xinyu, Zhu Sailan, Li Yuling
College of Agronomy, Henan Agricultural University, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Maize Crop Science, 63 Nongye Rd., Zhengzhou 450002, China.
Deptment of Biology and Molecular Biology, Montclair State University, Montclair, NJ 07043, USA.
Int J Mol Sci. 2017 Aug 24;18(9):1840. doi: 10.3390/ijms18091840.
Grain weight is one of the most important yield components and a developmentally complex structure comprised of two major compartments (endosperm and pericarp) in maize ( L.), however, very little is known concerning the coordinated accumulation of the numerous proteins involved. Herein, we used isobaric tags for relative and absolute quantitation (iTRAQ)-based comparative proteomic method to analyze the characteristics of dynamic proteomics for endosperm and pericarp during grain development. Totally, 9539 proteins were identified for both components at four development stages, among which 1401 proteins were non-redundant, 232 proteins were specific in pericarp and 153 proteins were specific in endosperm. A functional annotation of the identified proteins revealed the importance of metabolic and cellular processes, and binding and catalytic activities for the tissue development. Three and 76 proteins involved in 49 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were integrated for the specific endosperm and pericarp proteins, respectively, reflecting their complex metabolic interactions. In addition, four proteins with important functions and different expression levels were chosen for gene cloning and expression analysis. Different concordance between mRNA level and the protein abundance was observed across different proteins, stages, and tissues as in previous research. These results could provide useful message for understanding the developmental mechanisms in grain development in maize.
粒重是最重要的产量构成因素之一，并且是一个发育复杂的结构，由玉米（Zea mays L.）中的两个主要部分（胚乳和果皮）组成。然而，对于众多相关蛋白质的协同积累，人们了解甚少。在此，我们使用基于等压标签相对和绝对定量（iTRAQ）的比较蛋白质组学方法，来分析籽粒发育过程中胚乳和果皮的动态蛋白质组学特征。在四个发育阶段，共鉴定出这两个部分的9539种蛋白质，其中1401种蛋白质是非冗余的，232种蛋白质在果皮中特异表达，153种蛋白质在胚乳中特异表达。对已鉴定蛋白质的功能注释揭示了代谢和细胞过程以及结合和催化活性对组织发育的重要性。分别针对胚乳和果皮中的特异蛋白质，整合了参与49条京都基因与基因组百科全书（KEGG）通路的3种和76种蛋白质，这反映了它们复杂的代谢相互作用。此外，选择了4种具有重要功能且表达水平不同的蛋白质进行基因克隆和表达分析。与之前的研究一样，在不同蛋白质、阶段和组织中观察到mRNA水平与蛋白质丰度之间存在不同程度的一致性。这些结果可为理解玉米籽粒发育的调控机制提供有用信息。
