Tebyanian H, Karami A, Motavallian E, Aslani J, Samadikuchaksaraei A, Arjmand B, Nourani M R
Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Department of General Surgery, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Cell Mol Biol (Noisy-le-grand). 2017 Aug 15;63(7):46-51. doi: 10.14715/cmb/2017.63.7.8.
Pulmonary diseases cusecs a large portion of mortality in the world. There is no more cure for pulmonary diseases and many approaches are needed for finding ways to cure. Nowadays, implantation and drugs are only ways for curing those people who are facing with these diseases. Tissue engineering and regenerative medicine have been appeared as multidisciplinary field and also, they presents new therapeutic approaches for pulmonary diseases. One of these therapeutic approaches is decellularization which removes cellular but leaves intact important extracellular matrix (ECM) proteins and three-dimensional (3D) architecture and also, this approach has been studied for in-vitro and ex-vivo. In this study, we aimed to investigate a comparison of different concentrations of Triton X-100 and Sodium dodecyl sulfate detergents in lung decellularization in order to evaluate the effects of different concentrations and times of mentioned detergents on three dimensional and ECM proteins lung. Two detergents (Triton-X100 and Sodium dodecyl sulfat) were used with different concentrations for decellularizing rat lungs for maintaining of three-dimensional lung architecture and ECM protein compositions which have significant roles in differentiation and migration of stem cells. Results showed that SDS 0.05%, 0.1% and Triton-X100 0.1% could maintain 3D, elastin and collagen better than other concentrations in 24 and 48 h- decellularization. We concluded that these approaches can help to achieve three-dimensional architecture and extracellular matrix of lung with minimum destruction for next step such as recellularization and in-vivo study.
肺部疾病在全球死亡率中占很大比例。目前尚无治愈肺部疾病的方法，需要多种途径来寻找治愈方法。如今，植入和药物是治疗这些疾病患者的唯一方法。组织工程和再生医学已成为多学科领域，并且它们为肺部疾病提供了新的治疗方法。其中一种治疗方法是去细胞化，它去除细胞，但保留完整的重要细胞外基质（ECM）蛋白和三维（3D）结构，并且这种方法已在体外和离体条件下进行研究。在本研究中，我们旨在研究不同浓度的 Triton X - 100 和十二烷基硫酸钠去污剂在肺去细胞化过程中的比较，以评估上述去污剂不同浓度和时间对肺三维结构和 ECM 蛋白的影响。使用两种不同浓度的去污剂（Triton - X100 和十二烷基硫酸钠）对大鼠肺进行去细胞化，以维持对干细胞分化和迁移具有重要作用的肺三维结构和 ECM 蛋白组成。结果表明，在 24 小时和 48 小时的去细胞化过程中，0.05%、0.1%的 SDS 和 0.1%的 Triton - X100 比其他浓度能更好地维持三维结构、弹性蛋白和胶原蛋白。我们得出结论，这些方法有助于在最小程度破坏的情况下获得肺的三维结构和细胞外基质，以便进行下一步如再细胞化和体内研究。
