Nardi-Schreiber Atara, Gamliel Ayelet, Harris Talia, Sapir Gal, Sosna Jacob, Gomori J Moshe, Katz-Brull Rachel
Department of Radiology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
Nat Commun. 2017 Aug 24;8(1):341. doi: 10.1038/s41467-017-00364-3.
The dissolution-dynamic nuclear polarization technology had previously enabled nuclear magnetic resonance detection of various nuclei in a hyperpolarized state. Here, we show the hyperpolarization of P nuclei in important biological phosphates (inorganic phosphate and phosphocreatine) in aqueous solutions. The hyperpolarized inorganic phosphate showed an enhancement factor >11,000 (at 5.8 T, 9.3% polarization) in DO (T 29.4 s). Deuteration and the solution composition and pH all affected the lifetime of the hyperpolarized state. This capability opens up avenues for real-time monitoring of phosphate metabolism, distribution, and pH sensing in the live body without ionizing radiation. Immediate changes in the microenvironment pH have been detected here in a cell-free system via the chemical shift of hyperpolarized inorganic phosphate. Because the P nucleus is 100% naturally abundant, future studies on hyperpolarized phosphates will not require expensive isotope labeling as is usually required for hyperpolarization of other substrates.Real-time monitoring of phosphate metabolism and distribution in the live body without ionizing radiation is highly desirable. Here, the authors show dissolution-dynamic nuclear polarization technology can enable nuclear magnetic resonance detection of hyperpolarized P of important biological phosphates in aqueous solutions.
溶解动态核极化技术此前已能够对处于超极化状态的各种原子核进行核磁共振检测。在此，我们展示了水溶液中重要生物磷酸盐（无机磷酸盐和磷酸肌酸）中磷原子核的超极化。超极化的无机磷酸盐在D₂O（T₂为9.4 s）中显示出增强因子>11000（在5.8 T，极化率为9.3%）。氘代以及溶液组成和pH值均会影响超极化状态的寿命。这种能力为在无电离辐射的情况下实时监测活体中的磷酸盐代谢、分布和pH传感开辟了途径。在此，通过超极化无机磷酸盐的化学位移，在无细胞系统中检测到了微环境pH值的即时变化。由于磷原子核天然丰度为100%，未来对超极化磷酸盐的研究将无需像其他底物超极化通常所需的那样进行昂贵的同位素标记。非常需要在无电离辐射的情况下实时监测活体中的磷酸盐代谢和分布。在此，作者表明溶解动态核极化技术能够对水溶液中重要生物磷酸盐的超极化磷进行核磁共振检测。
