Akhi Mohammad Taghi, Ghotaslou Reza, Alizadeh Naser, Yekani Mina, Beheshtirouy Samad, Asgharzadeh Mohammad, Pirzadeh Tahereh, Memar Mohammad Yousef
Research Center of Infectious and Tropical Disease, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Bacteriology and Virology, School of Medicine, Tabriz University of Medical Sciences, Iran.
GMS Hyg Infect Control. 2017 Aug 17;12:Doc13. doi: 10.3205/dgkh000298. eCollection 2017.
is the most common anaerobic pathogen isolated from surgical site infections (SSIs). Metronidazole resistance is increasing and the mechanisms of resistance are not clear in some isolates. The aim of the present study was to investigate the metronidazole susceptibility prevalence, and detect genes in isolates from SSIs. This study included 100 surgery patients with signs and symptoms indicative of SSIs. Syringe aspiration of the infected site was used to collect specimens. All specimens were cultured on BBA (Brucella blood agar), KVLB (kanamycin-vancomycin laked blood), and BBE (Bacteroides bile esculin) agar. The MIC (minimum inhibitory concentration) of metronidazole was determined by the agar dilution method according to the Clinical and Laboratory Standard Institute (CLSI). Then the PCR method was used to determine the presence of the gene. In the present study, 26 were isolated from 100 SSIs specimens. Eight isolates were metronidazole resistant; the metronidazole MIC was 32 µg/mL for 7 isolates and 64 µg/mL for one isolate. All isolates were gene negative. The emergence of metronidazole-resistant limits the application of this drug for treatment and prophylaxis of SSIs. Thus, rapid identification of metronidazole-resistant is essential to restrict inappropriate, superfluous administration. In spite of various metronidazole resistance mechanisms other than that depending on the gene, detection of by PCR is unsuitable for identifying resistant isolates. Therefore, phenotypic methods are better to screen for and identify metronidazole-resistant .
是从手术部位感染（SSIs）中分离出的最常见厌氧病原体。甲硝唑耐药性正在增加，且某些分离株的耐药机制尚不清楚。本研究的目的是调查甲硝唑敏感性流行情况，并检测手术部位感染分离株中的基因。本研究纳入了100例有手术部位感染体征和症状的手术患者。通过注射器抽吸感染部位来采集标本。所有标本均在BBA（布鲁氏菌血琼脂）、KVLB（卡那霉素 - 万古霉素溶血血液琼脂）和BBE（拟杆菌胆汁七叶苷琼脂）上培养。根据临床和实验室标准协会（CLSI）的方法，采用琼脂稀释法测定甲硝唑的最低抑菌浓度（MIC）。然后使用PCR方法确定基因的存在情况。在本研究中，从100份手术部位感染标本中分离出26株。8株分离株对甲硝唑耐药；7株分离株的甲硝唑MIC为32μg/mL，1株为64μg/mL。所有分离株基因均为阴性。甲硝唑耐药菌的出现限制了该药物在手术部位感染治疗和预防中的应用。因此，快速鉴定甲硝唑耐药菌对于限制不适当、多余的用药至关重要。尽管除了依赖基因的耐药机制外，还有各种甲硝唑耐药机制，但通过PCR检测基因不适用于鉴定耐药分离株。因此，表型方法更适合筛选和鉴定甲硝唑耐药菌。
