载环丙沙星碳酸钙（CaCO）纳米粒子对常见骨髓炎细菌的抗菌作用。
The Antibacterial Effect of Ciprofloxacin Loaded Calcium Carbonate (CaCO) Nanoparticles Against the Common Bacterial Agents of Osteomyelitis.
机构信息
Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Curr Microbiol. 2023 Apr 8;80(5):173. doi: 10.1007/s00284-023-03234-y.
The present study aimed to investigate the biocompatibility, antibacterial/anti-biofilm effects of ciprofloxacin-loaded calcium carbonate (Cip- loaded CaCO) nanoparticles against the common organisms responsible for osteomyelitis. The antibacterial and biofilm inhibitory activities were studied by determination of minimum inhibitory concentrations (MICs) and minimum biofilm inhibitory concentrations (MBICs), respectively. Hemolytic effects were determined for studying hemocompatibility. The SDS-PAGE method was used to study the interaction of Cip- loaded CaCO with plasma proteins. The effects of Cip- loaded CaCO on the cell viability of human bone marrow mesenchymal stem cells (hBM-MSCs) was detected. The Cip- loaded CaCO nanoparticles were shown a significant antimicrobial effect at lower concentrations than free ciprofloxacin. No significant hemolytic effect was observed. The Cip- loaded CaCO nanoparticles have shown interaction with apolipoprotein A1 (28 kDa) and albumin (66.5 kDa). The viability of hBM-MSCs treated with Cip- loaded CaCO was more than 96%. Our results indicated that Cip-loaded CaCO nanoparticles had favorable in vitro compatibility with human red blood cells, antimicrobial effects, and low cytotoxicity.
本研究旨在探讨载环丙沙星碳酸钙（Cip-loaded CaCO）纳米颗粒的生物相容性、抗细菌/抗生物膜效应对常见引起骨髓炎的病原体的影响。通过测定最小抑菌浓度（MIC）和最小生物膜抑制浓度（MBIC），分别研究了其抗菌和生物膜抑制活性。通过测定溶血效应，研究了其血液相容性。采用 SDS-PAGE 方法研究了 Cip-loaded CaCO 与血浆蛋白的相互作用。检测了 Cip-loaded CaCO 对人骨髓间充质干细胞（hBM-MSCs）活力的影响。与游离环丙沙星相比，载 Cip 的 CaCO 纳米颗粒在较低浓度下表现出显著的抗菌作用。未观察到明显的溶血作用。Cip-loaded CaCO 纳米颗粒与载脂蛋白 A1（28 kDa）和白蛋白（66.5 kDa）有相互作用。用 Cip-loaded CaCO 处理的 hBM-MSCs 的活力大于 96%。我们的研究结果表明，载 Cip 的 CaCO 纳米颗粒与人红细胞具有良好的体外相容性、抗菌作用和低细胞毒性。
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