Kudo Y, Ito K, Miyakawa H, Izumi Y, Ogura A, Kato H
Brain Res. 1987 Mar 24;407(1):168-72. doi: 10.1016/0006-8993(87)91233-9.
Calcium-dependent fluorescence of a Ca2+ indicator (fura-2) loaded in the slice of guinea pig hippocampus was measured by a microscope/video-camera/photometry system. Tetanic stimulation of the perforant path (PP) or application of L-glutamate caused increment of the fluorescence from the dendritic and somatic layers of the granule cells in the dentate gyrus. Magnitude of the increment depended on the frequency and intensity of the PP-stimulation or on the dose of L-glutamate. 2-Aminophosphonovaleric acid, a glutamate-receptor antagonist, suppressed both PP-stimulus-induced and L-glutamate-evoked responses, while tetrodotoxin blocked the former only. Thus the fluorescence increment should represent an elevation of Ca2+ concentration in the postsynaptic cytoplasm of the granule cells.
通过显微镜/视频摄像机/光度测量系统测量了装载于豚鼠海马切片中的Ca²⁺指示剂（fura-2）的钙依赖性荧光。对穿通通路（PP）进行强直刺激或施加L-谷氨酸会导致齿状回颗粒细胞树突层和胞体层的荧光增强。荧光增强的幅度取决于PP刺激的频率和强度或L-谷氨酸的剂量。谷氨酸受体拮抗剂2-氨基磷酸戊酸可抑制PP刺激诱导的反应和L-谷氨酸诱发的反应，而河豚毒素仅阻断前者。因此，荧光增强应代表颗粒细胞突触后细胞质中Ca²⁺浓度的升高。
