Soares Scheila S, Souza Thamires K, Berté Francisco K, Cantarelli Vlademir V, Rott Marilise B
Programa de Pós-graduação em Microbiologia Agrícola e do Ambiente, Instituto de Ciências Básicas e da Saúde, UFRGS, Porto Alegre, RS, Brazil.
Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Sarmento Leite, 500, Porto Alegre, RS, 90050-170, Brazil.
Curr Microbiol. 2017 Dec;74(12):1461-1468. doi: 10.1007/s00284-017-1341-8. Epub 2017 Aug 24.
This study determined the occurrence of potentially pathogenic free-living amoebae (FLA) and bacteria associated with amoebae in air-conditioning cooling towers in southern Brazil. Water samples were collected from 36 cooling systems from air-conditioning in the state of Rio Grande do Sul, Brazil. The organisms were identified using polymerase chain reaction (PCR) and sequencing automated. The results showed that these aquatic environments, with variable temperature, are potential "hot spots" for emerging human pathogens like free-living amoebae and bacteria associated. In total, 92% of the cooling-tower samples analyzed were positive for FLA, and Acanthamoeba was the dominant genus by culture and PCR. Amoebal isolates revealed intracellular bacteria in 39.3% of them and all were confirmed as members of the genus Pseudomonas. The results obtained show the important role of cooling towers as a source of amoebae-associated pathogens.
本研究确定了巴西南部空调冷却塔中潜在致病性自由生活阿米巴（FLA）以及与阿米巴相关的细菌的存在情况。从巴西南里奥格兰德州的36个空调冷却系统中采集了水样。使用聚合酶链反应（PCR）和自动测序对这些生物进行鉴定。结果表明，这些温度多变的水生环境是自由生活阿米巴和相关细菌等新兴人类病原体的潜在“热点”。总共，92%的分析冷却塔样本对FLA呈阳性，棘阿米巴属是培养和PCR检测中的优势属。在39.3%的阿米巴分离株中发现了细胞内细菌，且所有这些细菌均被确认为假单胞菌属成员。所获得的结果表明冷却塔作为与阿米巴相关病原体来源的重要作用。
