Kämpfer Peter, Busse Hans-Jürgen, McInroy John A, Glaeser Stefanie P
Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
Institut für Mikrobiologie, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3345-3350. doi: 10.1099/ijsem.0.002115. Epub 2017 Aug 25.
A yellow-pigmented bacterial strain, JM-222T, isolated from the root tissue of field-grown cotton (Gossypium hirsutum) in Auburn, Alabama, USA, was studied for its taxonomic allocation. Cells of the isolate were rod-shaped and Gram-stain-negative. A blast analysis using the EzTaxon database showed that strain JM-222T shared highest 16S rRNA gene sequence similarity with the type strain of Flavobacterium endophyticum (98.9 %) followed by Flavobacterium qiangtangense (96.7 %). 16S rRNA gene sequence similarities to all other Flavobacterium species were below 95 %. The fatty acid profile of strain JM-222T consisted of the major fatty acids C15 : 0, iso-C15 : 0, iso-C15 : 0 2-OH/C16 : 1ω7c and iso-C17 : 0 3-OH. Major compounds in the polar lipid profile were phosphatidylethanolamine and several unidentified lipids. The quinone system consisted predominantly of menaquinone MK-6. The polyamine pattern consisted of the major compound sym-homospermidine and only minor amounts of other polyamines. These data and the differential biochemical and chemotaxonomic properties showed that the isolate JM-222T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium gossypii sp. nov. is proposed. The type strain is JM-222T (LMG 28821T=CCM 8610T).
从美国阿拉巴马州奥本市田间种植的棉花（陆地棉）根组织中分离出一株黄色色素细菌菌株JM-222T，并对其进行了分类学鉴定。该分离株的细胞呈杆状，革兰氏染色阴性。使用EzTaxon数据库进行的blast分析表明，菌株JM-222T与内生黄杆菌模式菌株的16S rRNA基因序列相似度最高（98.9%），其次是羌塘黄杆菌（96.7%）。与所有其他黄杆菌属物种的16S rRNA基因序列相似度均低于95%。菌株JM-222T的脂肪酸谱由主要脂肪酸C15:0、异C15:0、异C15:0 2-OH/C16:1ω7c和异C17:0 3-OH组成。极性脂质谱中的主要化合物是磷脂酰乙醇胺和几种未鉴定的脂质。醌系统主要由甲基萘醌MK-6组成。多胺模式由主要化合物对称高亚精胺和少量其他多胺组成。这些数据以及不同的生化和化学分类学特性表明，分离株JM-222T代表黄杆菌属的一个新物种，为此提出了黄杆菌棉属新种的名称。模式菌株为JM-222T（LMG 28821T=CCM 8610T）。
