Kämpfer Peter, Busse Hans-Jürgen, McInroy John A, Glaeser Stefanie P
Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, , A-1210 Wien, Austria.
Int J Syst Evol Microbiol. 2015 Jul;65(7):2179-2186. doi: 10.1099/ijs.0.000237. Epub 2015 Apr 9.
A yellow-pigmented bacterial strain, 91A-612(T), isolated from the geocarposphere (soil around the peanut) of very immature peanuts (Arachis hypogaea) in Alabama, USA, was studied for its taxonomic position. Cells of the isolate were rod-shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequence with the sequences of the type strains of the most closely related species showed that the strain belongs to the genus Chryseobacterium, showing the highest sequence similarities to the type strains of Chryseobacterium molle (98.4%), C. pallidum (98.3%) and C. hominis (97.8%). The 16S rRNA gene sequence similarities to the type strains of all other species of the genus Chryseobacterium were below 97.0%. The fatty acid profile of strain 91A-612(T) consisted of the major fatty acids iso-C15 : 0, summed feature 3 (iso-C15 : 0 2-OH/C16 : 1ω7c) and iso-C17 : 0 3-OH. Major compounds in the polar lipid profile were phosphatidylethanolamine and several unidentified lipids, including two lipids that did not contain a sugar moiety, an amino group or a phosphate group (L3, L8), and an aminolipid (AL1). The quinone system was composed mainly of MK-6. The polyamine pattern contained sym-homospermidine as the major compound and moderate amounts of spermidine and spermine. DNA-DNA hybridizations between strain 91A-612(T) and the type strains of C. molle, C. pallidum and C. hominis resulted in relatedness values well below 70%. These data and the differentiating biochemical and chemotaxonomic properties showed that isolate 91A-612(T) represents a novel species of the genus Chryseobacterium, for which we propose the name Chryseobacterium arachidiradicis sp. nov. (type strain 91A-612(T) = LMG 27814(T)= CCM 8490(T) = CIP 110647(T)).
从美国阿拉巴马州极不成熟的花生（落花生）的地下球层（花生周围的土壤）中分离出一株黄色色素细菌菌株91A - 612(T)，对其分类地位进行了研究。该分离株的细胞呈杆状，革兰氏染色阴性。将其16S rRNA基因序列与最密切相关物种的模式菌株序列进行比较，结果表明该菌株属于金黄杆菌属，与柔弱金黄杆菌（Chryseobacterium molle）模式菌株的序列相似性最高（98.4%），与苍白金黄杆菌（C. pallidum）模式菌株的序列相似性为98.3%，与人金黄杆菌（C. hominis）模式菌株的序列相似性为97.8%。与金黄杆菌属所有其他物种模式菌株的16S rRNA基因序列相似性均低于97.0%。菌株91A - 612(T)的脂肪酸谱由主要脂肪酸异C15 : 0、总和特征3（异C15 : 0 2-OH/C16 : 1ω7c）和异C17 : 0 3-OH组成。极性脂质谱中的主要化合物为磷脂酰乙醇胺和几种未鉴定的脂质，包括两种不含糖部分、氨基或磷酸基团的脂质（L3、L8）以及一种氨基脂质（AL1）。醌系统主要由MK - 6组成。多胺模式以对称高亚精胺为主要化合物，含有适量的亚精胺和精胺。菌株91A - 612(T)与柔弱金黄杆菌、苍白金黄杆菌和人金黄杆菌模式菌株之间的DNA - DNA杂交相关值远低于70%。这些数据以及区分性的生化和化学分类学特性表明，分离株91A - 612(T)代表金黄杆菌属的一个新物种，我们将其命名为花生根金黄杆菌（Chryseobacterium arachidiradicis）新种（模式菌株91A - 612(T) = LMG 27814(T)= CCM 8490(T) = CIP 110647(T)）。
