1 SFR Condorcet, Université de Reims Champagne-Ardenne, URVVC EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, 51687 Reims Cedex 2, France.
2 Agroécologie, AgroSup Dijon, CNRS, INRA, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France.
Mol Plant Microbe Interact. 2017 Dec;30(12):946-959. doi: 10.1094/MPMI-02-17-0036-R. Epub 2017 Oct 4.
Esca disease is one of the major grapevine trunk diseases in Europe and the etiology is complex, since several inhabiting fungi are identified to be associated with this disease. Among the foliar symptom expressions, the apoplectic form may be distinguished and characterized by sudden dieback of shoots, leaf drop, and shriveling of grape clusters in a few days that can ultimately induce the plant death. To further understand this drastic event, we conducted transcriptomic and metabolomic analyses to characterize responses of leaves during the period preceding symptom appearance (20 and 7 days before foliar symptom expression) and at the day of apoplexy expression. Transcriptomic and metabolomic analyses provide signatures for the apoplectic leaves and most changes concerning the metabolism of carbohydrates, amino acids, and phenylpropanoids. In deciphering glutathione-S-transferase (GST), its preferential location in phloem, correlated with the upregulation of GST genes and a decrease of the glutathione level, offers further support to the putative role of glutathione during apoplexy expression.
酒囊病是欧洲主要的葡萄树干病害之一，病因复杂，因为有几种栖息真菌被认为与这种病害有关。在叶片症状表现中，可以区分出中风型，并以枝条突然枯萎、叶片脱落和葡萄串在几天内枯萎为特征，最终可能导致植物死亡。为了进一步了解这一剧烈事件，我们进行了转录组和代谢组分析，以表征症状出现前（叶片症状表现前 20 天和 7 天）和中风表达当天叶片的反应。转录组和代谢组分析为中风叶片提供了特征，并对碳水化合物、氨基酸和苯丙烷代谢的大多数变化进行了描述。在解析谷胱甘肽-S-转移酶（GST）时，它在韧皮部的优先位置与 GST 基因的上调和谷胱甘肽水平的降低相关，这为谷胱甘肽在中风表达期间的可能作用提供了进一步的支持。
