Goufo Piebiep, Cortez Isabel
Centre for the Research and Technology of Agro-Environment and Biological Sciences, Universidade de Trás-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, Portugal.
Departamento de Agronomia, Universidade de Trás-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, Portugal.
Data Brief. 2020 Oct 28;33:106469. doi: 10.1016/j.dib.2020.106469. eCollection 2020 Dec.
The dataset presented in this paper comprises the masses of 208 lipid species and other compounds of lipid metabolism, and their levels in leaves of vines with brown wood streaking and grapevine leaf stripe, two symptomatic expressions of Esca complex disease (ESCA). Healthy, asymptomatic and symptomatic leaves were collected from the cultivar Malvasia Fina grown in a vineyard. The lipidome of these leaves was characterized using a platform consisting of an Ultrahigh Performance Liquid Chromatography and a Gas chromatography equipment coupled to a Q-Exactive Hybrid Quadrupole-Orbitrap high resolution/accurate Mass Spectrometer interfaced with a heated electrospray ionization source. The analysis permitted the detection of 158 molecular species of known identity and 50 species of unknown structural identity. The area counts of these molecular species is reported in the dataset, along with fold changes (log2-ratio), -values (Welch's two-sample -test), and -values (false discovery rate) from all pairwise comparisons among experimental groups. These statistical data are intended to serve as means of identification for lipid species whose levels were altered by the disease, and which could be used as biomarkers of symptom emergence and disease progression. Because of few studies on the subject, the association between modulation of lipid biosynthetic pathways and disease progression in grapevine has remained poorly understood. The analysis of the data described here has already provided new perspectives regarding the pathogenesis of ESCA leaf symptom formation. Reanalysis of these data would undoubtedly unravel some physiological roles played by lipids in the adaptation of vine plants to stressful conditions.
本文呈现的数据集包含208种脂质种类以及脂质代谢的其他化合物的质量，及其在患有褐木条纹病和葡萄叶条纹病（埃斯卡综合症（ESCA）的两种症状表现）的葡萄藤叶片中的含量。从一个葡萄园种植的玛尔维萨菲娜（Malvasia Fina）品种中收集了健康、无症状和有症状的叶片。使用由一台超高效液相色谱仪和一台气相色谱仪组成的平台对这些叶片的脂质组进行了表征，该平台与一台配备加热电喷雾电离源的Q-Exactive混合四极杆-轨道阱高分辨率/精确质谱仪相连。该分析检测出了158种已知身份的分子种类和50种结构身份未知的种类。数据集中报告了这些分子种类的面积计数，以及实验组间所有成对比较的倍数变化（log2比值）、p值（韦尔奇双样本t检验）和q值（错误发现率）。这些统计数据旨在作为鉴定那些因疾病而含量发生变化、可作为症状出现和疾病进展生物标志物的脂质种类的手段。由于关于该主题的研究较少，脂质生物合成途径的调节与葡萄疾病进展之间的关联仍未得到很好的理解。对这里描述的数据进行分析已经为ESCA叶片症状形成的发病机制提供了新的视角。对这些数据的重新分析无疑将揭示脂质在葡萄植株适应胁迫条件过程中所起的一些生理作用。
